紫菜薹子叶离体培养不定芽诱导频率的研究  

Study on Induction Rate of Cotyledon Adventitious Buds of Purple Cai-tai (Brassica campestris ssp.chinensis var.purpuraria) in vitro

作　　者：林小芳[1] 付明星[1] 刘乐承[1] 

机构地区：[1]长江大学园艺园林学院,湖北荆州434025

出　　处：《长江大学学报（自科版）（中旬）》2010年第1期60-62,66,共4页Journal of Yangtze University(Nature Science Edition)

基　　金：湖北省教育厅重点项目(2007ABA386)

摘　　要：为比较紫菜薹不同基因型之间的子叶不定芽诱导频率,以6个紫菜薹(Brassica campestris ssp.chinensis var.purpuraria)品种为试材,在不同的培养基上诱导不定芽,发现‘十月鲜’紫菜薹的子叶不定芽诱导频率最高。为确定‘十月鲜’紫菜薹子叶不定芽诱导最佳培养基,采用二次正交旋转组合设计研究了6-BA、NAA及AgNO3对‘十月鲜’子叶离体培养不定芽诱导频率的影响,发现3个因素对子叶不定芽诱导影响的主次关系为:AgNO3>6-BA>NAA,最佳培养基是MS+3.0 mg/L6-BA+1.5 mg/L NAA+5.0 mg/LAgNO3,不定芽诱导率达85.71%。For comparison of the induction rate of cotyledon adventitious buds between different genes of purple cai-tai(Brassica campestris ssp.chinensis var.purpuraria),6 cultivars of purple cai-tai was used as material,study on different combinations.It was found that among the 6 cultivars of purple cai-tai,Shiyuexian purple cai-tai obtained the highest induction rate of cotyledon adventitious buds.In order to determine the fittest combination of induction cotyledon adventitious buds of Shiyuexian purple cai-tai,the effects of 6-BA,NAA and AgNO3 on induction rate of cotyledon adventitious buds were studied by quadratic orthogonal rotary design method.The results showed that the relationship of the 3 factors on induction rate of cotyledon adventitious buds was: AgNO3 >6-BA>NAA.The best combination of inducing cotyledons adventitious buds was MS+3.0 mg/L 6-BA+1.5 mg/L NAA+5.0 mg/L AgNO3,and the induction rate of cotyledon adventitious buds was 85.71%.

关 键 词：紫菜薹(Brassica CAMPESTRIS ssp.chinensis var.purpuraria) 子叶 离体培养 植株再生 

分 类 号：Q813.1[生物学—生物工程]