影响日本结缕草成熟种子愈伤组织诱导率和出愈时间的相关因子研究  

Factors Affecting Callus Induction Rate and Formation Time of Mature Seed of Zoysia japonica

作　　者：刘雅丽[1] 费永俊[1] 曾会明[2] 

机构地区：[1]长江大学园艺园林学院,湖北荆州430025 [2]北京林业大学草坪研究所,北京100083

出　　处：《长江大学学报（自科版）（中旬）》2010年第1期63-66,共4页Journal of Yangtze University(Nature Science Edition)

基　　金：国家"863"计划(2006AA10Z132);北京林业大学博士点基金(20070417)

摘　　要：诱导率和出愈时间是愈伤组织诱导过程中的关键参数。本研究以日本结缕草(Zoysia japonica)成熟种子为材料,研究了研磨、接种方式和浸泡等因素对愈伤组织诱导率和出愈时间的影响。结果显示,在MS+2,4-D 5 mg/L+L-Pro 500 mg/L+蔗糖30 g/L+琼脂7 g/L培养基上,pH5.8时,研磨处理的结缕草种子愈伤组织诱导率远高于未研磨处理的;随机接种方式的愈伤组织诱导率高于机械接种方式的;浸泡72h后愈伤组织诱导率高。结果表明,在研磨情况下,日本结缕草种子浸泡72 h后,采取随机接种方式,培养第5～7 d即出现愈伤组织,第9～11 d愈伤组织发生达到高峰期,第14 d便可进行继代培养,愈伤组织诱导率达95.6%。The induction rate and callus formation time are the key parameters during the process of callus induction.Mature seeds of Japanese lawngrass(Zoysia japonica) were used as explants to investigate the effect of the factors,including grinding,immersion and the way of transferring explants on the medium,on callus induction rate and formation time.Results are as follows: when the culture medium for callus induction was MS+ 2,4-D 5 mg/L + L-Pro 500 mg/L + sucrose 30 g/L + agar 7 g/L,pH5.8,(1) the callus induction rate was significantly improved after seeds were ground;(2) with the way of transferring explants on medium at random,the induction rate increased;(3) the induction rate was higher when the mature seeds were immersed in water for 72 h.Taken together,these findings suggest that after the mature seeds were ground,immersed in water for 72 h and transferred on medium at random,a little callus appears from explants after seeds were on medium 5～7 d,the callus formation comes to peak at 9～11 d,callus can be in subculture after 14 d,and the callus induction rate are as high as 95.6%.

关 键 词：日本结缕草(Zoysia japonica) 愈伤组织 诱导率 出愈时间 

分 类 号：Q813.1[生物学—生物工程]