3种人抑制素α亚基N末端片段对hCG诱发排卵和胚泡着床的影响  

Effects of Inhibin α-Subunit-N-Terminal Fragments P^32,P^33 and P^29 on hCG-Induced Superovulation and Implatation

作　　者：张江红[1] 周文江[2] 李文伟 姜洁 程治平[3] 孙颖[4] 鲁桂琛[4] 

机构地区：[1]上海医科大学妇产科研究所,上海200011 [2]上海医科大学实验动物部,上海200032 [3]哈尔滨医科大学生理教研室,哈尔滨150086 [4]中国医学科学院药物研究所,北京100050

出　　处：《生殖与避孕》1999年第1期7-10,共4页Reproduction and Contraception

基　　金：国家计划生育委员会和黑龙江省教育委员会资助

摘　　要：我们合成了抑制素α亚单位N末端1－32氨基酸（32肽．P23）．[Tyr]－1－32氨基酸（33肽，P33）和37－65氨基酸（29肽，P29）片段。本实验观察这三个片段对排卵和着床影响。在PMSG－HCG诱发小鼠超排卵实验中．P29具有显著的刺激超排卵作用．P29组排卵率是单纯hCG组的两倍（P＜0．001）。在着床实验中．利用大鼠双子宫角的特点进行自身对照实验。在胚泡着床前期，对妊娠大鼠一侧子宫角注入100μl生理盐水作为对照组，另一侧注入100μl（300ng）抑制素α亚基片段。结果除P29一例外．均完全阻断胚泡着床（P＜0．001）。Human inhibin α - subunit-N-terminal 1-32 amino acid (peptide 32. P32 ). [Tyr]-1-32 (P33) and 37-65 (P29) were synthesized. In this edperiment. the effects of 3 fragments on ovulation and implantation of embryo were observed. PMSG-hCC-induced superovulation mouse model was used for the testing of ovulation. Among 3 fragmentls. only P20 showed significant stimstimulating effect on ovulation. the ovulation rate in P29 group was twice as much as in control (hCGinduced superovulation rate ) (P＜ 0. 001 ). For implantation experim,ent.100μl vehicle was injected one side uterus of pregnant rat before implation and the other side uterus received 100 μl (300 ng ) of inhibin frament P29. P32 and P33 respectively. The result showed that except for one case in group of P29. they all absolutely block the implantation of embryo (P＜0.01).

关 键 词：抑制素 α亚基片段 抗着床 排卵 胚泡着床 

分 类 号：R979.2[医药卫生—药品] R169[医药卫生—药学]