禽流感H11N9亚型病毒东方白鹳分离株的基因特征  

Genome Properties of Avian Influenza H11N9 Virus from Oriental White Stork

作　　者：张兰兰[1] 柴洪亮[1] 曾祥伟[1] 张进[1] 郭亮[1] 华育平[1] 

机构地区：[1]东北林业大学,哈尔滨150040

出　　处：《东北林业大学学报》2010年第2期76-78,82,共4页Journal of Northeast Forestry University

基　　金：野生鸟类禽流感病毒生态分布研究及病毒库建立(SKLVBF200906)

摘　　要：根据已知禽流感病毒(AIV)的8个基因序列设计合成12对特异引物,通过反转录-聚合酶链式反应(RT-PCR)技术,分别成功扩增出分离于东方白鹳(Ciconia boyciana)的1株H11N9亚型禽流感病毒的全基因序列,将其分别克隆到pMD18-T载体后进行序列测定。同时将8个基因片段与GenBank发表的相应序列进行比较,绘制各基因的进化树。结果表明:所克隆到的8个片段均包含相应病毒基因的完整开放阅读框架,长度分别为2280、2274、2151、1692、1497、1413、759、838bp核苷酸,分别编码759、757、716、563、498、470、252/97、230/121个氨基酸。该毒株HA有7个潜在糖基化位点;在HA裂解位点序列为PAIASR↓GLFG,无连续的多个碱性氨基酸插入,具有典型的低致病性禽流感病毒特征。根据该毒株与参考毒株的序列比较分析结果推测,该分离株可能是不同亚型禽流感病毒通过互相基因交换所形成的基因重组体。Reverse transcription-polymerase chain reaction（RT-PCR）was used to amplify the full-length cDNAs of an avian influenza virus（AIV）isolated from an Oriental White Stork（Ciconia boyciana）.Furthermore,the cDNAs were cloned into pMD18-T and then sequenced.These sequences were compared with the corresponding sequences published in GenBank,and phylogenetic trees were drawn.Results show that eight cDNA fragments all contain the whole open reading frame of corresponding gene,which are composed of 2280,2274,2151,1692,1497,1413,759 and 838 nucleotides,respectively encoding 759,757,716,563,498,470,252/97 and 230/121 amino acids residues.There are seven potential glycosylation sites in HA gene.The amino acid sequence of the cleavage site between HA1 and HA2 is PAIASR↓GLFG,with the typical characteristics of the low pathogenic avian influenza virus.The results of comparative sequence analysis indicate that the gene of the isolate is recombinant.

关 键 词：禽流感病毒 H11N9亚型 东方白鹳分离株 序列分析 

分 类 号：S858[农业科学—临床兽医学]