白三叶×高加索三叶草F1代茎段离体培养器官发生的研究  

Study on organogenesis of stem segments in vitro of F1 generation of Trifolium ambiguum×T. repens

作　　者：黄帆[1] 王明玖[1] 何丽君[2] 陈丽丽[1] 

机构地区：[1]内蒙古农业大学生态环境学院,内蒙古呼和浩特010019 [2]内蒙古农业大学农学院,内蒙古呼和浩特010019

出　　处：《草业科学》2010年第5期85-90,共6页Pratacultural Science

基　　金：国家自然科学基金(30660118);内蒙古草业研究所草业专项(20071923-1.5)

摘　　要：以白三叶Trifoliumrepens×高加索三叶草T.ambiguum未成熟胚离体培养产生的F1代无菌苗的茎段为外植体,进行不定芽途径的直接器官发生培养。筛选适宜的诱导、分化及生根培养基,研究不同质量浓度激素组合对体细胞器官发生的影响,为建立扩繁体系及之后的回交试验奠定基础。结果表明:MS+2,4-D 0.1 mg/L、6-BA2 mg/L为适宜的诱导培养基,MS+NAA0.5 mg/L、6-BA1 mg/L、KT 1 mg/L为适宜的分化培养基,不添加任何激素的1/2 MS培养基为适宜的生根培养基。Stems of the hybrid F1 generation of Trifolium ambiguum×T.repens from immature embryos in vitro was used as the explants to culture the adventitious buds through the way of direct organogenesis.The suitable induction,differentiation and rooting medium were selected and the effect on organogenesis with different concentrations of hormone combinations was studied in order to prepare for the establishment of propagations system and subsequent backcross experiment.The results showed that the MS＋2,4-D 0.1 mg/L＋6-BA 2 mg/L was the appropriate induction media,the MS＋NAA 0.5 mg/L＋6-BA 1 mg/L＋KT 1 mg/L was the appropriate differentiation medium,and the 1/2MS medium which had no any hormones was the suitable rooting medium.

关 键 词：高加索三叶草 白三叶 杂交F1代 组织培养 器官发生 

分 类 号：S541.2[农业科学—作物学]