抗纤灵冲剂对单侧输尿管梗阻及再通后大鼠尿微量蛋白的影响  

Effects of "Kang Xian Ling Decoction" on Urinary Microprotein in Rats after Unilateral Ureteral Obstruction and Recanalization

作　　者：吴锋[1,2,3] 沈丽萍 张新志[1,2,3] 黄迪[1,2,3] 董飞侠[1,2,3] 何立群[1,2,3] 

机构地区：[1]上海中医药大学附属曙光医院,上海201203 [2]肝肾疾病病证教育部重点实验室,上海201203 [3]上海市高校中医内科E-研究院,上海201203 [4]上海市杨浦区中医医院,上海200041

出　　处：《时珍国医国药》2010年第5期1038-1040,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.30873259);上海市优秀学科带头人培养计划(No.08XD14039);上海市教育委员会E-研究院建设计划(No.E03008);上海市高校创新团队项目

摘　　要：目的研究单侧输尿管梗阻(UUO)及再通后大鼠尿微量蛋白的变化和抗纤灵对尿微量蛋白变化的影响。方法建立大鼠UUO模型120只和UUO再通模型90只,随机分为正常组、假手术组、模型组、抗纤灵组和科素亚组。于造模后各时间点检测大鼠尿转铁蛋白(TRF)、尿视黄醇结合蛋白(RBP)、尿β2-微球蛋白(β2-M)。结果在UUO模型中,模型组各时间点TRF、RBP、β2-M较正常组和假手术组明显升高(P<0.01),并随梗阻时间的延长逐渐升高。在UUO再通模型中,各时间点TRF、RBP、β2-M较正常组和假手术组明显升高(P<0.01),并随梗阻时间的延长逐渐降低。与模型组比较,抗纤灵能明显降低两模型中TRF、RBP、β2-M。结论抗纤灵冲剂能减少UUO模型和UUO再通模型中的尿微量蛋白尿,改善两种模型中的肾损伤。Objective To explore the effects of ＂Kang Xian Ling Decoction＂ （KXL） on urinary microprotein in rats after unilateral ureteral obstruction and recanalization.Methods 120 male Sprague-Dawley rats in UUO model and 90 male Sprague-Dawley rats in UUO recanalization model were randomly divided into 5 groups：Normal group,Shame-operated group,Model group,KXL group,and Lostartan group.Urinary transferrin （TRF）,retinol-blinding protein （RBP） and β2-microglobuli （β2-M） were tested weekly.Results In UUO model,the levels of TRF,RBP,β2-M in Model group were increased,compared with Normal group and Shame-operated group,and it had significant difference（P〈0.01）.In UUO model,the levels of TRF,RBP,β2-M in Model group were increased compared with Normal group and Shame-operated group（P〈0.01）,but they were decreased.Compared with Model group,TRF,RBP,β2-M contents in KXL group were decreased significantly.Conclusion Kang Xian Ling Decoction can decrease urinary microprotein in rats after UUO and UUO recanalization,and improve renal fibrosis.

关 键 词：抗纤灵冲剂 单侧输尿管梗阻 单侧输尿管梗阻后再通 尿微量蛋白 

分 类 号：R285.5[医药卫生—中药学]