鱼腥草蛋白酶抑制剂的分离和纯化  

The Extraction and Purification of Trypsin Inhibitor from Houttuynia cordata Thunb

作　　者：付煊赫[1] 刘同祥[2] 张宗申[1] 

机构地区：[1]大连工业大学生物与食品工程学院,辽宁大连116034 [2]中央民族大学中国少数民族传统医学研究中心,北京100081

出　　处：《时珍国医国药》2010年第5期1047-1048,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.30130220)

摘　　要：目的以鱼腥草为材料,研究鱼腥草蛋白酶抑制剂(HCTI)的分离提取工艺。方法 HCTI的最佳粗提条件是采用pH7.0的双蒸水为提取溶剂,在体积比为1:30,45℃条件下浸提2.0h,提取2次。硫酸铵部分沉淀后,再经DEAE-52离子交换层析柱纯化。结果粗提液经45%～60%饱和度的硫酸铵沉淀比活力最高,经过DEAE-52离子交换层析柱梯度洗脱后,检测到HCTI活性峰。结论用DEAE-52离子交换层析柱可分离纯化HCTI,SDS-聚丙烯酰胺凝胶电泳,计算出该HCTI的分子量约为28KDa。Objective To study the isolation and purification procedure of TI from Houttuynia cordata Thunb.Methods The optimal criteria of TI extraction were 45 ℃,30 times of solvent volume,pH 7.0 and 2 h.The combination of ammonium sulphate precipitation and DEAE-cellulose column chromatography was used to purify the TI.Results The specific activity peak of TI occurred in the part of 45%～60% （NH4）2SO4 precipitation,and was consequently purified by DEAE-cellulose column gradient elution,and the activity of TI could be detected.Conclusion The HCTI can be purified with DEAE-cellulose column,and the molecular weight of HCTI is determined by SDS-PAGE as 28 KDa.

关 键 词：胰蛋白酶抑制剂 鱼腥草 分离纯化 

分 类 号：R284.2[医药卫生—中药学]