中药莪术中抗肿瘤成分莪术醇人工抗原的合成与鉴定  

Synthesis and Identification of Artificial Antigen for Curcumol,An Antitumor Compounds from Rhizoma Zedoariae

作　　者：杨新平[1] 陈旭[1,2] 李芳耀[1] 阮方[1] 林家逊[1] 钟海雁[2] 

机构地区：[1]桂林医学院.药学院,广西桂林541004 [2]中南林业科技大学,湖南长沙410004

出　　处：《时珍国医国药》2010年第5期1054-1056,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.30960498);广西自然科学基金(No.桂科自0447101);广西科技攻关与新产品试制(No.桂科攻0992003A-26)

摘　　要：目的合成莪术醇全抗原并加以鉴定。方法先用莪术醇与琥珀酸酐为原料,以三氯甲烷为溶剂,4-二甲基氨基吡啶(DMAP)作催化剂,反应合成半抗原莪术醇琥珀酸单酯,然后,用1-乙基-3(3-二甲基氨基丙基)碳二亚胺法将莪术醇琥珀酸单酯偶联到牛血清白蛋白,合成了莪术醇的人工抗原,并用激光解析飞行质谱(MALDI-TOF-MS)进行了鉴定,利用质谱特征求得了偶联物中莪术醇与牛血清白蛋白的量,计算出结合比。结果成功地合成了莪术醇人工抗原,其结合比为19.6。结论用1-乙基-3(3-二甲基氨基丙基)碳二亚胺偶联法可合成莪术醇的完全抗原。Objective To synthesize and identify the artificial antigen of curcumol.Methods Firstly,a hapten of curcumol,curcumol-hemisucinate（Cur.-HS） was synthesized using material of curcumol to react with succinic anhydride in chloroform and trace 4-dimethylaminopyridine（DMAP） as catalyst.Then the hapten was conjugated to bovine serum albumin（BSA） using 1-ethyl-3-（3-dimethylaminopropyl）carbodiimide,（EDC） method and the characterization of the antigen was examined by MALDI-TOF-MS,and the conjugation ratio of curcumol to BSA was determined with MALDI-TOF-MS.Results The artificial antigen was successfully synthesized and identified by MALDI-TOF-MS.The conjugation ratio of curcumol to BSA was 19.6.Conclusion The artificial antigen of curcumol is successfully synthesized by EDC method.

关 键 词：莪术 莪术醇 1-乙基-3(3-二甲基氨基丙基)碳二亚胺 人工抗原 莪术醇琥珀酸单酯 

分 类 号：R284.1[医药卫生—中药学]