育阴灵冲剂对自然流产模型母胎界面Th1/Th2型细胞因子及妊娠结局的影响  

Impacts of Yuyinling Infusion on Th1/Th2 Cytokines of Maternal-Fetal Interface and Pregnancy Outcome in Mischarge Model

作　　者：韩延华[1] 李丹丹[2] 高新源[2] 王洋[2] 唐艳[2] 

机构地区：[1]黑龙江中医药大学妇科教研室,黑龙江哈尔滨150040 [2]黑龙江中医药大学

出　　处：《世界中西医结合杂志》2010年第5期391-393,共3页World Journal of Integrated Traditional and Western Medicine

基　　金：黑龙江省科技攻关项目(No.GC07C34505)

摘　　要：目的探讨中药育阴灵冲剂对自然流产模型母胎界面Th1/Th2型细胞因子及妊娠结局的影响。方法建立正常妊娠模型CBA/J×BALB/C与自然流产模型CBA/J×DBA/2,并将自然流产模型CBA/J孕鼠随机分为三组(每组10只):中药组于妊娠第1天灌服育阴灵冲剂(2.35 g/mL);西药组于妊娠第4天(着床期)腹腔注射0.5 mg/kg CsA;模型组于妊娠第1天灌服0.2 mL生理盐水;另设正常妊娠模型CBA/J孕鼠10只(正常组),于妊娠第1天灌服0.2 mL生理盐水。孕13 d时观察4组孕鼠的胚胎吸收率,并采用RT-PCR测定各组母胎界面蜕膜组织中Th1型(IL-12、IFN-γ)/Th2型(IL-4、IL-10)细胞因子转录水平。结果与模型组比较,育阴灵冲剂能够升高母胎界面局部Th2型而降低Th1型细胞因子转录水平,并显著降低自然流产模型胚胎吸收率(P<0.01),与腹腔注射环孢素效果相当。结论育阴灵冲剂能够调控母胎界面局部Th1/Th2型细胞因子转录,形成维持正常妊娠所需的Th2型免疫偏倚,从而诱导母胎免疫耐受,改善其妊娠预后。Objective To investigate the impacts of yuyinling infusion on Th1/Th2 cytokines of maternal-fetal interface and pregnancy outcome in mischarge model.Methods The pregnant mice of mischarge CBA/J model were randomized into three groups（10 mice in each group）.In Chinese medicine group,yuyiling infusion（2.35 g/mL）was administered on the 1st day of pregnancy.In western medicine group,0.5 mg/lg CsA was injected intraperitoneally on the 4th day of pregnancy（implantation period）.In model group,0.2ml normal saline was administered in perfusion on the 1st day of pregnancy.Additionally,a normal pregnancy model of CBA/J with 10 pregnant mice was set up（normal group）,in which,0.2 mL normal saline was administered in perfusion on the 1st day of pregnancy.The embryonic absorption rate was observed in 13 days of pregnancy in 4 groups and RT-PCR assay was adopted to determine the cytokine transcription levels of Th1（IL-12,IFN-γ）/ Th2（IL-4,IL-10）type in maternal-fetal interface of each group.Results Compared with model group,yuyinling infusion increased the cytokine transcription level of Th2,decreased that of Th1 cytokines in local maternal-fetal interface and significantly reduced the absorption rate of mischarge model（P〈0.05）,which were similar to the results of intraperitoneal injection of cyclosporine.Conclusion Yuyinling infusion can regulate the cytokine transcription of Th1/Th2 types in local maternal-fetal interface and results in Th2 immune bias required for the maintenance of normal pregnancy so as to induce maternal-fetal immune tolerance and improve pregnant outcome.

关 键 词：育阴灵冲剂 母胎界面 TH1/TH2型细胞因子 疾病模型 动物 小鼠 

分 类 号：R714.21[医药卫生—妇产科学]