滑桃树愈伤组织与内生真菌共培养的代谢产物研究  

Studies on the Secondary Metabolites of Co-culture of the Calli of Trewia nudiflora and Its Endophtic Strain

作　　者：吴欣[1] 鲁春华[1] 

机构地区：[1]厦门大学生命科学学院福建省药物工程实验室,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2010年第3期406-409,共4页Journal of Xiamen University：Natural Science

基　　金：国家自然科学基金(30430020);国家杰出青年基金(30325044)

摘　　要：建立滑桃树愈伤组织与其内生真菌(Fusariumsp.WXE)的共培养体系,并对共培养的次生代谢产物进行研究.研究结果表明,滑桃树愈伤组织对内生真菌的生长具有明显的促进作用.采用核磁共振、质谱等波谱技术对从共培养提取物中分离到的3个化合物进行结构测定,确定它们分别为(16α)-17-hydroxy-en-tatisan-19-oic acid methyl ester(1),(16α)-16,17-dihydroxy-ent-atisan-19-oic acid methyl ester(2)和β-谷甾醇(3).The co-cultures of calli of Trewia nudiflora L.and its endophytic fungus Fusarium sp.WXE were established.The secondary metabolites of the co-culture were studied.The result showed that the growth of Fusarium sp.WXE was obviously induced by the calli of T.nudiflora.Three compounds were isolated from the co-culture extract,and their structures were elucidated to be（16α）-17-hydroxy-ent-atisan-19-oic acid methyl ester（1）,（16α）-16,17-dihydroxy-ent-atisan-19-oic acid methyl ester（2） and β-sitosterol（3） according to their spectroscopic analyses including NMR and MS.

关 键 词：滑桃树 愈伤组织 共培养 内生真菌 

分 类 号：Q945[生物学—植物学] Q939.9