利用SSR和SFP标记分析不同抗瘟性水稻品种的多态性  

Polymorphism analysis of different rice varieties with SSR and SFP marks

作　　者：黄玲[1] 黄红梅[2,3] 肖应辉[1] 刘雄伦[1] 刘二明[3] 戴良英[3] 

机构地区：[1]湖南农业大学农学院,湖南长沙410128 [2]湖南农业大学生物科学技术学院,湖南长沙410128 [3]湖南农业大学生物安全科学技术学院,湖南长沙410128

出　　处：《湖南农业大学学报（自然科学版）》2010年第3期267-271,共5页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家"973"计划项目(2006CB101904);国家"948"计划项目(2006-G61);国家自然科学基金项目(30871335);湖南省教育厅项目(20060537004)

摘　　要：利用914对引物(848对SSR引物和66对SFP引物),对籼型广谱持久抗瘟水稻品种湘资3150和籼型感病品种CO39的基因组多态性进行研究.结果表明,881对引物(815对SSR引物和66对SFP引物)有扩增产物,扩增率为96.39%.共筛选到220对多态性引物,多态性检出率为24.10%.SSR标记的多态性检出率为23.58%,低于SFP标记的多态性检出率(30.30%).不同染色体间多态性检出率差异较大,第10染色体的供试引物多态性检出率最高,为30%;第9染色体的多态性检出率最低,为14.55%.不同碱基数目基序的标记间多态性检出率有较大差异,其中4碱基重复型的多态性检出率最高,为27.96%,其后依次为2碱基重复型、复合型、3碱基重复型和单碱基重复型.914 marks（848 SSR and 66 SFP）were used to find the genomic polymorphism between the blast resistant rice variety Xiangzi3150 and the blast susceptible variety CO39.The results showed that expected bands were amplified from 96.39% of 881 markers（815 SSR and 66 SFP）,among which 220 polymorphic tags were identified and 24.10% were.The polymorphism rate of the SSR mark（23.58%） was lower than that of the SFP mark（30.30%）.The rate of polymorphic marks on the 12 chromosomes was different.Chromosome 10 had the highest rate（30.00%） and chromosome 9 had the lowest rate（14.55%）.SSR marks with different units also showed a significant difference in polymorphism rate.The SSR marks with tetranucleotide repeats had the highest polymorphism rate（27.96%）,followed by the repetitive dinucleotide,repetitive trinucleotide and repetitive single-nucleotide tags,respectively.

关 键 词：水稻 微卫星标记 SFP标记 稻瘟病抗性 多态性 

分 类 号：S511.035.3[农业科学—作物学] Q78[生物学—分子生物学]