BWMK1互作基因BWIP4对粳稻的转化  

Transformation of the BWMK1-interacting gene BWIP4

作　　者：刘玲[1,2] 高佳[1,2] 潘素君[2] 胡亚军[2] 刘雄伦[2] 王国梁[2,3] 戴良英[1,2] 

机构地区：[1]湖南农业大学生物安全科学技术学院,湖南长沙410128 [2]湖南农业大学水稻基因组学实验室,湖南长沙410128 [3]俄亥俄州州立大学植物病理系,俄亥俄州哥伦布43210

出　　处：《湖南农业大学学报（自然科学版）》2010年第3期272-275,共4页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家自然科学基金项目(30871335);国家"863"计划项目(2008AA10Z106)

摘　　要：通过酵母双杂交体系,获得了7个BWMK1互作基因BWIP1～BWIP7.从中选取BWIP4作进一步的功能分析,构建了BWIP4-RNAi表达载体pANDA-BWIP4和超表达载体NCGR-1300-BWIP4,利用农杆菌介导法转化粳稻日本晴,获得了35个干涉株系和136个超表达转基因株系,对经PCR、GUS检测的阳性植株进一步进行RT-PCR检测,干涉株系中BWIP4的表达量低于日本晴,而超表达株系中BWIP4的表达量高于日本晴.BWMK1,a first reported rice MAPK gene,was transcriptionally activated by blast fungus infection and wounding.To make a further understanding about the BWMK1-mediated defense signaling,we identified 7 BWMK1 interacting genes by yeast two-hybrid screening,designate BWIP1~BWIP7,and selected BWIP4 for further functional characterization.Through Agrobacterium mediated transformation method,we generated overexpression and RNAi transgenic plant using a japonica cultivar nipponbare as a receptor.The GUS assay and PCR results showed that the target gene had been intergrated into receptor.Further expression analysis was confirmed by RT-PCR.These transgenic lines provided us a good material for in-deepth function analysis of BWIP4.

关 键 词：BWMK1 BWIP4 农杆菌介导法 水稻 

分 类 号：S511.035.3[农业科学—作物学]