猪肠毒素大肠杆菌F4受体的微卫星标记筛选  

Screening of microsatellite markers on enterotoxigenic Escherichia coli F4(K88) receptor in different swine breeds

作　　者：蒋隽[1,2] 刘小兰 马海明[2] 柳小春[2] 何俊[2] 黄生强[2] 张细权[1] 

机构地区：[1]华南农业大学动物科学学院,广东广州510642 [2]湖南农业大学动物科学技术学院,湖南长沙410128 [3]湖南省洞口县成人中等专业学校,湖南邵阳422300

出　　处：《湖南农业大学学报（自然科学版）》2010年第3期321-325,共5页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家自然科学基金青年项目(30800607);全国优秀博士论文作者专项基金(200972)

摘　　要：从猪13号染色体选取与肠毒素大肠杆菌(ETEC)F4受体基因连锁的8个微卫星座位,研究不同猪种间的遗传特性,并分析不同基因型与F4受体黏附表型的关系.结果表明,2个地方猪种(五指山猪和沙子岭猪)和2个外来猪种(大约克猪和杜洛克猪)在8个基因座均具有高度多态性,杂合度为0.6117～0.7500,多态信息含量在0.5749以上.对大约克猪和沙子岭猪的F4黏附性和微卫星的关联研究表明,在微卫星座位S0223和SW207,2个猪种不同基因型在F4ab和F4ac血清型黏附表型上差异显著或极显著,可能F4ab和F4ac受体就位于这2个位点之间.这2个微卫星座位可望作为ETECF4抗性基因的遗传标记.8 microsatellite markers linked with enterotoxigenic Escherichia coli （ETEC） F4 receptor gene were selected from chromosome 13 of swine to analyze difference in heredity between the genotype and F4 receptor adhesive appearance. Results showed that the 8 microsatellite markers were highly polymorphic and the heterozygosities ranged from 0.611 7 to 0.750 0. The value of PIC was over 0.574 9 and the hereditary diversity of Chinese pig breeds was much higher than oversea＇s.There were signficiant difference between genotypes and F4ab and F4ac adhesive in microsatellite S0223 and SW207 in the two breeds, which indicated the F4ab and F4ac receptors gene are located in the two loci. S0223 and SW207 can thus be potentially used as a genetic marker for E. coli F4 resistive gene.

关 键 词：肠毒素大肠杆菌 微卫星座位 基因频率 遗传差异 猪 

分 类 号：S828.99[农业科学—畜牧学] Q343.17[农业科学—畜牧兽医]