舒巴坦钠和EDTA使产ESBLs鸡大肠杆菌恢复对抗菌药物敏感性  

Recovery of antibacterial sensitivity of ESBLs-producing fowl E.coli by sulbactam and EDTA

作　　者：刘保光[1] 刘建华[1] 吴华[1] 陈玉霞[1] 胡功政[1] 徐利纳[1] 陈燕杰[1] 孟春萍[1] 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002

出　　处：《湖南农业大学学报（自然科学版）》2010年第3期326-329,共4页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家自然科学基金项目(30771624)

摘　　要：为测定舒巴坦钠、膜通透性促进剂(EDTA)对抗菌药物体外抗菌活性的影响,采用微量肉汤稀释法,测定10种抗菌药物及其与舒巴坦钠、EDTA、舒巴坦钠+EDTA联用对产ESBLs鸡大肠杆菌的最小抑菌浓度(MIC).结果表明,舒巴坦钠和EDTA可以增强部分抗菌药物对产ESBLs鸡大肠杆菌的抗菌活性,产ESBLs菌对常用第三代头孢噻呋、头孢噻肟的耐药率高于80%,对氟喹诺酮类、多西环素、氟苯尼考、阿米卡星、磷霉素的耐药率高达100%,对第四代头孢头孢吡肟的敏感率为100%;对加舒巴坦钠的头孢噻肟、氟苯尼考、阿米卡星及磷霉素的耐药率低于未加舒巴坦钠的单方药物,耐药率分别为0、88.9%、88.9%、0;对加EDTA的加替沙星、多西环素、氟苯尼考、阿米卡星及磷霉素的耐药率低于未加EDTA的单方药物,耐药率分别为30%、11.1%、22.2%、11.1%、0;多数菌株对加舒巴坦钠+EDTA的阿米卡星、磷霉素、氟苯尼考、头孢噻呋的耐药率均较仅加舒巴坦钠或EDTA的低,耐药率均为0.To determine effect of sulbactam and EDTA on antibacterial activity of antimicrobial drugs in vitro,MIC of 10 antimicrobial agents alone and their respective combinations with sulbactam,EDTA and sulbactam＋ EDTA against ESBLs-producing E.coli,were determined by using broth microdilution method.The results showed that sulbactam and EDTA can enhance antibacterial activity of parts of antimicrobial agents,the resistant rate of ESBIs-producing isolates to third generation cephalosporin ceftiofur,cefotaxime was higher than 80% and the resistant rates to fluoroquinolones,doxycycline,florfenicol,amikacin and fosfomycin were up to 100%.All the ESBIs-producing isolates were sensitive to cefepime.The resistant rate to third generation cephalosporin cefotaxime,florfenicol,amikacin and fosfomycin combinations with sulbactam,were lower than that to antibacterial drugs alone,is 0,88.9%,88.9%,0 respectively.The resistant rate to gatifloxacin,doxycycline,florfenicol,amikacin and fosfomycin combinations with EDTA,were lower than that to antibacterial drugs alone,is 30%,11.1%,22.2%,11.1%,0 respectively.The resistant rates of most strains to sulbactom＋EDTA amikacin,fosfomycin,florfenicol and ceftiofur combinations were much lower than those combinations either only sulbactam or EDTA and were all 0.

关 键 词：舒巴坦钠 EDTA 超广谱Β-内酰胺酶 鸡大肠杆菌 敏感性 

分 类 号：S831.7[农业科学—畜牧学] R818.051[农业科学—畜牧兽医]