蕨麻猪生长激素基因的克隆及多态性分析  

Cloning and Polymorphism Analysis of Growth Hormone Gene in Juema Swine

作　　者：李军成[1,2] 路彩霞[2] 景志忠[1] 

机构地区：[1]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室,农业部兽医公共卫生重点实验室,甘肃省动物寄生虫病重点实验室,甘肃兰州730046 [2]广西农业职业技术学院,广西南宁530007

出　　处：《安徽农业科学》2010年第12期6227-6230,共4页Journal of Anhui Agricultural Sciences

基　　金：农业部农业结构调整重大技术研究专项(04-10-03B);国家高技术发展计划(863)项目(2003AA241111)

摘　　要：[目的]探索蕨麻猪生长激素基因与其生理学和生物学特性的关系。[方法]从蕨麻猪血液细胞中提取基因组DNA,通过PCR技术扩增出蕨麻猪生长激素基因序列,进行碱基组成多态性和突变分析。[结果]蕨麻猪生长激素(pGH)基因序列全长1671bp,有5个外显子和4个内含子。外显子1、2、3、4和5的碱基数依次是10、161、117、162和201bp,内含子1、2、3和4的碱基数分别是244、210、192和277bp。除第1外显子和第1内含子碱基剪切有明显的例外,其余全部符合GT-AG规则;蕨麻猪生长激素基因4种碱基含量依次为G>C>T>A。(G+C)含量和(A+T)含量分别在62%和37%以上。蕨麻猪编码区第4外显子在+1046位处A→G,为错义突变,其余均为同义突变。蕨麻猪生长激素基因编码的前体蛋白含216个氨基酸,成熟蛋白是由190个氨基酸残基组成的一个分泌性蛋白;二级结构中含有6个α螺旋结构,三级结构是结构松散的球状蛋白,含有6个α螺旋前区。[结论]为蕨麻猪生长激素基因序列的研究提供了参考依据和借鉴。[Objective] To explore the relation between growth hormone gene sequence with physiology and biology characteristic of Juema swine.[Method] The genome DNA was extracted from the blood of Juema swine.The growth hormone gene sequence of Juema swine was amplifyied through PCR and analyzed.[Result] The length of growth hormone gene sequence in Juema swine was 1 671 bp,and the gene sequence contained 5 exons and 4 introns.The base number of exons 1,2,3,4 and 5 were 10,161,117,162 and 201 bp,respectively,and the base number of introns 1,2,3 and 4 was 244,210,192 and 277 bp,respectively.GT-AG regulation can be found all the gene sequence except the first exon and intron.The order of four kinds bases content in Juema swine＇s growth hormone gene are G〉C〉T〉A.The contents of（G ＋ C）and（A ＋ T）are more than 62% and 37%,respectively.The forth exon of Juema swine showed missense mutation A→G in ＋1 046,others were all synonymous mutation.The precursor protein coding by Juema pig＇s growth hormone gene contained 216 amino acids,and maturation protein was a secretory protein consisting of 190 amino acid residue.It contained 6 α-helix helical structures in the secondary structure.It was a globular protein with loose tertiary structure,which contained 6 α-helix anterior regions.[Conclusion] The research provide reference and basis for researching the growth hormone gene sequence of Juema swine.

关 键 词：蕨麻猪 生长激素基因 多态性 

分 类 号：S828[农业科学—畜牧学]