猪NCOA1基因PCR-RFLP多态性分析  

Polymorphism Analysis of NCOA1 Gene in Pigs by PCR-RFLP

作　　者：侯永刚[1] 闫守庆[2] 何晓波[1] 柏蒙蒙[1] 孙泰雷[1] 吴明明[1] 孙金海[1,2] 

机构地区：[1]青岛农业大学动物科技学院,山东青岛266109 [2]吉林大学畜牧兽医学院,吉林长春130062

出　　处：《安徽农业科学》2010年第12期6235-6236,共2页Journal of Anhui Agricultural Sciences

基　　金：转基因生物新品种培育重大专项课题研究计划项目(20082X-08006-003);国家自然科学基金项目(30871778);山东省泰山学者建设工程专项经费

摘　　要：[目的]研究猪NCOA1基因的PCR-RFLP多态性,为猪的分子育种和标记辅助选择提供理论依据。[方法]以81头山东寿光六和原种猪场的长白猪为研究对象,采取常规酚-氯仿抽提法从猪耳组织提取基因组DNA,以基因组DNA为模板进行PCR扩增获得440bp的NCOA1基因目的片段,利用限制性内切酶RsaⅠ对PCR产物进行酶切和琼脂凝胶电泳检测,并计算各等位基因频率和基因型频率。[结果]琼脂糖电泳检测酶切产物出现3种基因型:AA型(1条带:440bp)、AB型(3条带:440、282、158bp)、BB型(2条带:282、158bp);基因型频率分别为0.54、0.43和0.03;A和B2个等位基因的基因频率分别为0.76和0.24。[结论]该研究猪群中,A为优势等位基因,AA基因型频率较高。[Objective] The research aimed to study the polymorphism of NCOA1 gene in pigs by PCR-RFLP.[Method] Using 81 Landrace pigs from Liuhe pig breeding farm as research objects,the genome DNA was extracted from ear tissue of pigs by using conventional Phenol-chloroform extraction method.The genomic DNA was used as template to make PCR amplification and a 440 bp target fragment was obtained.PCR amplification products were digested by restriction nuclease Rsa Ⅰ.The agarose gel electrophoresis detection was made.And the gene frequency and the genotype frequency were calculated.[Result] The agarose gel electrophoresis detection showed that the enzyme digestion products had three genotypes of AA（440 bp）,AB（440,282,158 bp）and BB（282,158 bp）and the genotype frequencies of AA,AB and BB were 0.54,0.43 and 0.03 respectively.The frequencies of allele A and B were 0.76 and 0.24 respectively.[Conclusion] In the studied pig herd,A is the dominant allele and the genotype frequency of AA is higher.

关 键 词：猪 NCOA1基因 PCR-RFLP 多态性 

分 类 号：S828[农业科学—畜牧学]