经前舒颗粒对经前期综合征肝气郁证大鼠大脑皮层雌激素受体表达的影响  

Effects of Jingqianshu Granule on the expression of estrogen receptor(ER) of PMS model rats with liver-qi stagnation

作　　者：冯玉[1] 张惠云[1] 

机构地区：[1]山东中医药大学,济南250355

出　　处：《中药药理与临床》2010年第2期71-73,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：山东中医药大学中医药经典理论教育部重点实验室;山东中医药大学中药质量分析国家中医药管理局三级实验室国家自然科学基金资助项目(No.90709009);国家重点基础研究发展规划(973计划)项目(No.2006CB504804)

摘　　要：目的:研究经前舒颗粒对经前期综合征(Premenstrual Syndrome,PMS)肝气郁证大鼠大脑皮层雌激素受体(estrogen receptor,ER)表达水平的影响。方法:束缚造模法复制PMS肝气郁证大鼠模型,旷场实验对模型评价,RT-PCR和Western blot方法分别检测大鼠大脑皮层ERα、ERβ的mRNA及蛋白的表达。结果:经前舒颗粒给药后大鼠旷场实验得分显著升高,大脑皮层中ERα、ERβ的mRNA和蛋白表达均显著降低。结论:大鼠大脑皮层中ER是经前舒颗粒的作用靶点之一,经前舒颗粒可能通过降低大脑皮层中ERα、ERβ的表达而发挥疗效。Objectives：To research the effect of jingqianshu granule on the expression of estrogen receptor（ER） of premenstrual syndrome （PMS） model rats with liver-qi stagnation in cerebral cortex regions,and to investigate its mechanisms of action in treatment of PMS liver-qi stagnation. Methods：Female Sprague-Dawley rats were introduced for model rats with PMS liver-qi stagnation by bondaging the limbs,and evaluated the model by open-field method. We analyzed the expression of ERα and ERβ in cerebral cortex by the method of Western blot and RT-PCR. Results：The expression of ERα and ERβ in cerebral cortex of model rats increased obviously comparing with the normal control group. Comparing to the model group,the ERα and ERβ decreased after treated with Jingqianshu graulne. Conclusion：The Jingqianshu granule can down-regulate the ERα and ERβ expression levels of model rats in cerebral cortex,which may be one of the mechanisms treating PMS with liver-qi stagnation. The expressions changes of the ERα and ERβ are in close relation to PMS with liver-qi stagnation.

关 键 词：经前期综合征肝气郁证 雌激素受体 经前舒颗粒 

分 类 号：R285.5[医药卫生—中药学]