家兔体内黄艾美耳球虫的发育史观察  

Endogenous Development of Eimeria flavescens in Domestic Rabbit

作　　者：王岩[1] 赵金凤[1] 王强[1] 张龙现[1] 宁长申[1] 林昆华[2] 

机构地区：[1]河南农业大学牧医工程学院,郑州450002 [2]中国农业大学动物医学院,北京100094

出　　处：《动物学杂志》2010年第3期91-97,共7页Chinese Journal of Zoology

基　　金：河南省重大公益性科研项目(No.81100912300);国家科技部自然资源平台项目(No.2005DKA21104)

摘　　要：为了解家兔(Oryctolagus curiculus)黄艾美耳球虫(Eimeria flavescens)的内生发育史,用不同接种剂量感染20只无球虫兔,采用常规石蜡切片技术进行研究。共观察到4代裂殖生殖和1代配子生殖。每一代裂殖生殖阶段都含有2种类型的裂殖体:粗型和细型。第1代裂殖生殖发生于感染后60～72h,主要位于空肠的腺上皮;第2代裂殖生殖发生于感染后84h,位于盲肠和结肠的腺上皮;第3代裂殖生殖发生于感染后96～120h,位于盲肠和结肠的绒毛上皮;第4代裂殖生殖发生于感染后144～153h,位于结肠和盲肠的腺上皮。感染后167h,开始配子生殖阶段,成熟卵囊出现于感染后215h。本研究中对于裂殖生殖代数划分与文献报道不同,并观察到2种形态的裂殖体。In order to understand the endogenous development of Eimeria flavescens,20 coccidia-free rabbits （Oryctolagus curiculus）were orally inoculated with E. flavescens sporulated oocysts and killed under anesthesia at intervals for collecting intestinal tissue samples. The samples were used for conventional paraffin section and H. E staining. Four asexual generations and one sexual generation. Two types of meronts and merozoites were found in each asexual generation：type A meronts gave rise to a smaller number of thick and short merozoites, while the type B meronts produced lots of thin and long merozoites. The first generation meronts were found in the crypts of the jejunum 60 - 72 h after inoculation （a. i. ）. The second generation meronts were found in the crypts of the large intestine 84 h after inoculation. The third generation meronts developed in the villi of the large intestine 96 - 120 h after inoculation. The fourth generation meronts were observed in the crypts of the large intestine 144 - 153 h after inoculation. The gamogony took place in large intestine 167 h after inoculation. The mature oocysts were found 215 h after inoculation.

关 键 词：家兔 内生发育 组织学 黄艾美耳球虫 

分 类 号：S858.291[农业科学—临床兽医学]