PDCD5在系统性红斑狼疮患者外周血单个核细胞中表达的初步研究  

Expression of programmed cell death 5 in peripheral blood mononuclear cells from patients with systemic lupus erythematosus

作　　者：赵丽娜[1] 赵杰[1] 贺宝玲[1] 张秀军[1] 

机构地区：[1]华北煤炭医学院,河北唐山063000

出　　处：《山东医药》2010年第20期22-24,共3页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(30671092)

摘　　要：目的研究程序性死亡因子5(PDCD5)在系统性红斑狼疮(SLE)患者外周血单个核细胞(PBMC)中的表达情况,探讨其可能作用机制。方法应用免疫细胞化学和蛋白免疫印迹法检测45例SLE活动期患者、22例稳定期患者和50例健康对照者PBMC中PDCD5蛋白的细胞定位和表达情况。结果SLE活动期患者PDCD5蛋白的阳性表达率高于正常对照组(P<0.01);活动期患者PDCD5蛋白的表达水平高于稳定期患者和正常对照,稳定期患者高于正常对照(P<0.01)。SLE活动期患者PBMC中PDCD5的表达与SLE疾病活动指标SLEDAI、补体C3、抗ds-DNA抗体滴度具有相关性。结论PDCD5可能作为淋巴细胞凋亡的正性调控基因,在SLE发生和发展中具有重要意义。Objective To study the expression of programmed cell death 5（PDCD5） in peripheral blood mononuclear cells（PBMC） from patients with systemic lupus erythematosus（SLE）,and to investigate the role of PDCD5 in the onset of SLE.Methods The localization and expression of PDCD5 in PBMC from 45 active SLE patients,22 inactive SLE patients and 50 healthy controls.Results PDCD5 protein was expressed widely in PBMC.The positive staining of PDCD5 was mainly found in cytoplasm.The positive rate of PDCD5 from active SLE patients was significantly higher than that in normal controls（P0.01）.The expression level of PDCD5 in active SLE patients was significantly higher than that of inactive SLE patients and that of normal controls.PDCD5 expression level of inactive SLE patients was significantly higher than that of normal controls（P0.01）.Significant correlation was found between the PDCD5 expression in PBMC from active SLE patients and clinical parameters such as SLEDAI,C3 and anti ds-DNA antibody.Conclusion PDCD5 plays an important role in the pathogenesis of human SLE.

关 键 词：系统性红斑狼疮 程序性死亡因子5 凋亡 外周血单个核细胞 

分 类 号：R592.324[医药卫生—老年医学]