越橘离体快繁体系的优化  

The optimization of bilberry rapid micropropagation in vitro culture

作　　者：段祖安[1] 李建华[1] 赵艳燕[1] 王洪利[1] 侯立群[2] 

机构地区：[1]山东农业大学林学院,山东泰安271018 [2]山东省林业科学研究院,山东济南250014

出　　处：《南京林业大学学报（自然科学版）》2010年第3期168-170,共3页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：山东省农业良种工程项目(鲁科农字[2005]99号)

摘　　要：以越橘(Vaccinium ssp.)茎段为外植体,改良WPM为基本培养基,对影响越橘离体快繁的因素进行分析。结果表明:3月份采集越橘作为外植体、暗处理22h、pH为4.2,改良WPM添加ZT(1mg/L)和TDZ(0.2mg/L)时,能够迅速建立起初代培养体系。促进越橘丛生芽萌发的最佳培养基为改良WPM+ZT(1.5mg/L)。This paper studied the factors affecting bilberry rapid micropropagation with improved WPM as basic culture medium and stem segment of bilberry as explant in vitro culture. The results showed that initial culture system was quickly created when the explants collected stem segment of bilberry in March was treated in dark at pH 4. 2 for 22 h and WPM was modified by adding ZT（1 mg/L） and TDZ（0. 2 mg/L）. The induced differentiation of bilberry multiple shoot clumps were verified with the improved WPM ＋ ZT （1. 5 mg/L） by orthogonal experiment.

关 键 词：越橘 外植体 快繁体系 

分 类 号：Q948[生物学—植物学]