消油剂对马粪海胆污染效应的影响  

Effect of dispersant and No.0 diesel oil pollution on sea urchin,Hemicentrotus pulcherrimus

作　　者：吕福荣[1,2] 熊德琪[1] 

机构地区：[1]大连海事大学环境科学与工程学院,辽宁大连116026 [2]大连大学环境与化学工程学院,辽宁大连116622

出　　处：《海洋环境科学》2010年第3期328-331,共4页Marine Environmental Science

基　　金：国家自然科学基金资助项目(40476046/D0608)

摘　　要：以马粪海胆(Hemicentrotus pulcherrimus)为测试对象,比较了0号柴油分散液(WAFs)与加入消油剂后的乳化液(dis-WAFs)的24、48、72、96 h急性毒性效应,并研究了不同浓度的柴油乳化液对马粪海胆消化腺超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性的影响。实验结果表明:0号柴油分散液对马粪海胆幼胆的半致死浓度分别为18.2、15.5、11.5、9.5 mg/L;0号柴油乳化液对马粪海胆幼胆的24、48、72、96 h半致死浓度分别为11.7、9.1、7.4、5.1 mg/L。暴露相同时间时,0号柴油乳化液的毒性大于0号柴油分散液。亚致死浓度的dis-WAFs污染对抗氧化酶活性影响存在剂量-效应关系。同一剂量组随着污染时间的延长,SOD和CAT的活力表现为先诱导后抑制的趋势,受污染个体在污染解除之后,其SOD和CAT酶活性得到恢复。SOD和CAT可以作为海洋底栖环境石油烃污染监测的潜在的生物标志物之一。The acute toxicities of No.0 diesel oil water-accommodated fractions（WAFs） and emulsion（dis-WAFs） on sea urchin,Hemicentrotus pulcherrimus were studied and the effect of No.0 diesel oil emulsion on activity of（SOD） and in digestive gland were investigated in this paper.The results showed that：24,48,72,96 h LC50 values of WAFs were 18.2,15.5,11.5,9.5 mg/L,and those of dis-WAFs were 11.7,9.1,7.4,5.1 mg/L,respectively.For the groups treated with the same time,dis-WAFs is greater than WAFs.The results also showed that with prolonging exposure time,the SOD and CAT activity are induced first,and then reduce gradually.The dose-effect relationship was in 13d sample;SOD and CAT activity of all concentration groups are recovered to the corresponding control level after the recovery experiment.According to the data,SOD and CAT could be one of biomarkers to monitor the benthic marine oil pollution.

关 键 词：海胆 0号柴油分散液(WAFs) 0号柴油乳化液(dis-WAFS) 超氧化物歧化酶(SOD) 过氧化氢酶(CAT) 

分 类 号：R994.6[医药卫生—毒理学]