银杏达莫对糖尿病肾病大鼠肾脏的保护作用  

Effects of Ginkgo Bilobate Extract on the Expression of Vascular Endothelial Growth Factor of Diabetic Nephropathy in-Rats

作　　者：张学彬[1] 孙芳[1] 薛凌宇[1] 

机构地区：[1]泰山医学院附属医院肾内科,山东泰安271000

出　　处：《河北医学》2010年第5期513-516,共4页Hebei Medicine

摘　　要：目的:探讨银杏达莫对糖尿病肾病(DN)大鼠肾组织血管内皮生长因子(VEGF)mRNA及蛋白质表达的影响。方法:SD大鼠分为对照组、DN组、银杏达莫治疗组,链脲菌素腹腔注射诱导DN大鼠模型,诱导成功后银杏达莫治疗组给予银杏达莫经胃管灌服。试验12周末检测大鼠体重、血糖、尿肌酐,24h尿白蛋白。采用RT-PCR检测VEGFmRNA及免疫组化检测蛋白质的表达。结果:DN组大鼠体重、血糖、尿白蛋白,VEGFmRNA及蛋白质的表达,肾小球细胞数,细胞外基质(ECM)聚积均高于对照组(P<0.01)。与DN组相比,银杏达莫治疗组大鼠体重、血糖、尿白蛋白,肾脏VEGFmRNA及免疫组化检测蛋白质的表达,ECM聚积显著降低(P<0.01或P<0.05)。尿VEGF与尿白蛋白呈正相关(P<0.05),与尿肌酐亦呈正相关(P<0.05)。结论:VEGF表达增加可能参与DN的发病机制。银杏达莫可减轻DN大鼠体重、血糖,减少尿白蛋白的排泄,抑制肾小球系膜增殖及硬化,降低VEGFmRNA及蛋白质的表达。Objective：To investigate the effects of ginkgo bilobate extract on the expression of renal vascular endothelial growth factor（ VEGF）mRNA and protein of diabetic nephropathy（DN） in rats.Method：SD rats were divided into control group,diabetic nephropathy group（DN group）,ginkgo bilobate extract treated group.DN was induced by peritoneal injection of streptozotocin（STZ）,with or without treatment by gastric perfusion of ginkgo bilobate extract daily until 12 weeks after the experiment.The body weights,blood,urine,24 hour total urinary albumin were measured.The expression of VEGF mRNA was determined by reverse transcription-polymerise chain reaction（RT-PCR） and the protein was detected by immunohistochemical staining.Result：The body weight,blood glucose,urinary albumin,expression of VEGF mRNA and protein,glomerular cellularity and extracellular matrix（ ECM） increased significantly in nephritic rats without treatment with Ginkqo-damole as compared to those in control rats（ P 0.0l）.However,the body weight,blood glucose,urinary albumin,expression of VEGF mRNA and protein,ECM decreased significantly in ginkgo bilobate extract treated nephritic rats as compared to thosein nephritic rats without treatment with Ginkqo-damolon （P 0.05）.The positive correlations were found between urinary levels of VEGF and urinary albumin（P0.05）,and between urinary levels of VEGF and urine creatinine（P0.05）.Conclusion：The overexpression of VEGF may participate in the pathogenesis of diabetic nephropathy.Ginkgo bilobate extract could contribute to decrease body weights,blood glouse,urinary albumin excrection,inhibit glomerular cellularity and ECM proliferation,downregulate the-expression of VEGF mRNA and protein.

关 键 词：糖尿病肾病 银杏达莫 血管内皮生长因子 

分 类 号：R587.2[医药卫生—内分泌]