黏病毒抗性蛋白A基因启动子区单核苷酸多态性与慢性丙型病毒性肝炎患者干扰素治疗反应的关系  

Relationship of single nucleotide polymorphism in MxA gene promoter regions and response to interferon treatment in patients with chronic hepatitis C virus infection

作　　者：宋红波[1] 武俊香[1] 贾因棠[1] 

机构地区：[1]山西医科大学第一医院感染科,太原030001

出　　处：《中国药物与临床》2010年第6期619-622,共4页Chinese Remedies & Clinics

基　　金：山西省出国留学回国人员基金(2009-52)

摘　　要：目的探讨α-干扰素(IFN-α)诱导黏病毒抗性蛋白A(MxA)基因启动子区-88(G/T)及-123(C/A)位点的单核苷酸多态性(SNP)与慢性丙型病毒性肝炎患者干扰素治疗效果间的关系。方法应用聚合酶链反应(PCR)及限制性片段长度多态性(RFLP)方法检测123例慢性丙型肝炎患者和188名健康人群的MxA基因启动子-88(G/T)及-123(C/A)位点的基因型,并探讨了慢性丙型肝炎患者-88(G/T)及-123(C/A)位点的SNP与干扰素抗病毒治疗反应之间的关系。结果MxA基因启动子区-88/-123位点各基因型在慢性丙型肝炎患者组与健康对照组中的分布频率差异无统计学意义;MxA基因启动子区-88位点为GT基因型的慢性丙型肝炎患者经干扰素治疗后获得持续病毒学应答(SVR)率显著高于GG基因型患者(P=0.009);而MxA基因启动子区-123位点各基因型对慢性丙型肝炎患者的IFN治疗反应无显著影响(P=0.096)。结论MxA基因启动子区-88GT基因型的患者对IFN治疗反应性好于GG基因型,因此,MxA-88位点的基因型可作为预测慢性丙型肝炎患者IFN疗效的参考指标之一。而MxA-123位点基因型与慢性丙型肝炎患者IFN疗效无相关性。Objective To investigate the relationship of single nucleotide polymorphisms （SNP） in MxA gene promoter region-88 and-123 loci and the treatment response to interferon-α in patients with chronic HCV infection. Methods Genotypes of the MxA promoter-88 （G/T） and-123 （C/A） loci were examined by polymerase chain reaction and restriction fragment length polymorphism （PCR-RFLP） in 123 patients with HCV infection and 188 controls, and the relationship between SNP polymorphisms in chronic HCV and response to interferon antiviral therapy was explored. Results The distribution of genotypes over MxA gene promoter regions-88/-123 loci was not significantly different between patients with chronic hepatitis C virus infection and healthy controls. Patients with GT genotypes at loci-88 at MxA gene promoter region had an obviously stronger sustained virologic response （SVR） than those with GG genotype （P=0.009）. There was no significant difference in IFN therapeutic response among patients with different genotypes at the loci-123 at the MxA promoter region （P=0.096）. Conclusion Patients with-88 GT genotypes at MxA gene promoter region seemed to be better responders to IFN treatment than those with GG genotype. Therefore, MxA-88 may be considered as an indicators for predicting IFN therapeutic response in patients with chronic hepatitis C. Genotype at MxA-88 loci was not shown to hanve any correlation with IFN efficiency.

关 键 词：肝炎 丙型 慢性 多态性 单核苷酸 MXA 

分 类 号：R512.63[医药卫生—内科学]