再生障碍性贫血患者死亡受体5的表达及意义  

Expression and significance of death receptor 5 in aplastic anemia

作　　者：韩丽[1] 张秀莲[1] 张伟华[1] 范星火[1] 陈玮[1] 侯素敏[1] 刘晨[1] 

机构地区：[1]山西医科大学第一医院血液科,太原030001

出　　处：《中国药物与临床》2010年第6期648-650,共3页Chinese Remedies & Clinics

摘　　要：目的通过检测再生障碍性贫血(简称再障)患者骨髓单个核细胞肿瘤坏死因子相关凋亡诱导配体(TRAIL)的死亡受体5(deathreceptor5,DR5)mRNA的表达,探讨DR5诱导的凋亡在诱导再障造血干细胞凋亡中的作用。方法采用实时定量反转录聚合酶链反应(RT-PCR)对12例再障(再障组)、11例再障治疗后达缓解(缓解组)、12名健康人(对照组)骨髓单个核细胞DR5mRNA的表达进行检测。结果①再障组、缓解组、对照组DR5mRNA均有表达。②再障组DR5mRNA的表达高于缓解组、对照组,差异有统计学意义(P<0.05)。③缓解组与对照组间DR5mRNA的表达差异无统计学意义(P>0.05)。结论DR5mRNA在再障患者骨髓单个核细胞中表达增高,提示DR5介导的细胞凋亡可能在再障的发病机制中发挥一定的作用。Objective To investigate the expression of death receptors（DR5） of TNF-related apoptosis inducing ligand （TRAIL） in the bone marrow mononuclear cells in aplastic anemia patients, and to approach its effects on apoptosis of haemopoietic stem cells in aplastic anemia. Methods Reverse transcriptase polymerase chain reaction （RTPCR） was used to detect the gene expressions of DR5 mRNA in mononuclear cells of bone marrow from 12 cases of aplastic anemia （AA group）, 11 AA cases with complete remission （CR group）, and 12 normal controls （control group）. Results ① DR5 mRNA was expressed in AA group, CR group and control group. ②The level of DR5 mRNA expression in AA group was higher than that in CR group and control group （P〈0.05）. ③There is no statistical difference in DR5 mRNA expression between CR group and control group （P〉0.05）. Conclusion The highest level of DR5 mRNA expression as found in mononuclear cells in patients with aplastic anemia suggested that DR5 may be playing an important role in pathogenesis of aplastic anemia.

关 键 词：贫血 再生障碍性 基因表达 肿瘤坏死因子诱骗受体 

分 类 号：R556[医药卫生—血液循环系统疾病]