更昔洛韦体外抗人巨细胞病毒的实验研究  

Inhibitory effect of Ganciclovir on human cytomegalovirus in vitro

作　　者：顾绍庆[1] 顾小海[2] 陈慧娟[3] 李继安[2] 叶亮英[2] 郑意[2] 

机构地区：[1]江苏大学附属医院儿科,江苏镇江212001 [2]江苏大学临床医学院,江苏镇江212001 [3]江苏大学附属医院神经内科,江苏镇江212001

出　　处：《江苏大学学报（医学版）》2010年第3期199-202,I0001,共5页Journal of Jiangsu University：Medicine Edition

基　　金：江苏省自然科学基金资助项目(BK2008238)

摘　　要：目的:探讨更昔洛韦体外抑制人巨细胞病毒(human cytomegalovirus,HCMV)的作用方式。方法:根据病毒复制周期设计3种作用模式来观察更昔洛韦对HCMV的抑制作用:模式Ⅰ将病毒与细胞混合后,再加入药物;模式Ⅱ将药物与细胞混合后,再加入病毒;模式Ⅲ将药物与病毒混合后再加入细胞。通过观察细胞病变效应,用噻唑蓝(MTT)比色法检测细胞存活率,以确定该药物治疗指数。结果:更昔洛韦对人胚成纤维细胞半数中毒浓度(TC50)为1 685.23 mg/L。在模式Ⅰ中,更昔洛韦对HCMV半数抑制浓度(IC50)为35.255 mg/L,治疗指数为47.8;在模式Ⅱ和Ⅲ中,更昔洛韦对HCMV无明显抑制作用。结论:更昔洛韦对HCMV感染细胞后显示出显著的抑制作用,且随药物浓度的增加其作用逐渐增强,存在明显的量效关系。同时,体外实验显示,更昔洛韦对人胚成纤维细胞感染HCMV无预防作用。Objective： To study the inhibitory effect of Ganciclovir（GCV） on human cytomegalovirus（HCMV） in vitro.Methods： According to viral replication cycle,three antiviral methods were designed to observe the anti-HCMV effects of Ganciclovir： Method I,Confluent cell monolayers were infected with HCMV.After some hours,it was replaced with the medium containing different served drug.Method Ⅱ,human embryo fibroblast cells were treated with the prearranged drug,Then 100 TCID50 of virus were added to these cells.Method Ⅲ,HCMV were incubated in GCV medium.Virus-drug mixed medium was added to the cells finally.By means of observing cytopathic effect and adopting MTT colorimetric assay for cell survival rate,their therapeutic index was calculated.Results： Median toxic concentration（TC50） of GCV on human embryo fibroblast cells was 1 685.23 mg/L.By method Ⅰ,the 50% inhibitory concentrations（IC50） of GCV for HCMV were 35.255 mg/L.By methodⅡand Ⅲ,GCV showed no obvious anti-virus effects.Conclusion： The above datas in vitro experimentation revealed that GCV can resist HCMV,and the inhibition effect would be strengthened with the increasing dose of GCV.It showed clear-dose connection.But GCV had no activity for guarding against human embryo fibroblast cells′ infection by HCMV in vitro.

关 键 词：更昔洛韦 人巨细胞病毒 细胞病变效应 噻唑蓝比色法 

分 类 号：R965[医药卫生—药理学]