预处理条件对石斛兰叶片原生质体分离的影响  

Impact of Pretreatment Conditions on the Protoplast Isolation of Dendrobium leaf

作　　者：罗丽华[1] 严虹羽[1] 林尤河[1] 韩宏丽[1] 

机构地区：[1]海南大学热带作物种质资源保护与开发利用教育部重点实验室,海南海口570228

出　　处：《南方园艺》2010年第3期10-12,共3页Southern Horticulture

摘　　要：试验研究了不同预处理条件对石斛兰叶片原生质体分离的影响,结果表明:预处理可以提高原生质体的产量,石斛兰叶片原生质体分离最佳预处理方法为:12 h黑暗条件萎蔫处理,0.4mmol/L CaCl2浸泡16 h,0.5 mol/L甘露醇浸泡16 h。The effects of different pretreatment conditions on Dendrobium leaf protoplast isolation were studied.The results showed that pretreatments can increase protoplast yield,the best pretreatment methods were wilting treatment in dark for 12 h,soaking treatment with 0.4 mol/L CaCl2 and 0.5 mol/L mannitol treatment for 16 h.

关 键 词：石斛兰 原生质体分离 预处理 

分 类 号：S968.42[农业科学—水产养殖]