引江济太对太湖水体碱性磷酸酶动力学参数的影响  

Influence of water diversion from Yangtze River to Taihu Lake on kinetic parameters of alkaline phosphatase activity in Taihu Lake subzones

作　　者：路娜[1,2] 胡维平[1] 邓建才[1] 翟淑华[3] 周小平[3] 陈效民[2] 

机构地区：[1]中国科学院南京地理与湖泊研究所湖泊与环境国家重点实验室,江苏南京210008 [2]南京农业大学资源与环境学院,江苏南京210095 [3]太湖流域水资源保护局,上海200434

出　　处：《水科学进展》2010年第3期413-420,共8页Advances in Water Science

基　　金：水体污染控制与治理科技重大专项资助项目(2008ZX07101-012);水利部太湖流域水资源保护局引江济太资助项目(TH1901);国家自然科学基金资助项目(40901252);江苏省科技厅基础研究计划资助项目(BK2007749);中国科学院南京地理与湖泊研究所所长基金资助项目(NIGLAS2007-01)~~

摘　　要：采用野外定点采样、室内同步分析水体碱性磷酸酶活性及水化学指标的方法,研究引江济太调水对太湖各湖区水体碱性磷酸酶最大反应速率(vmax)、米氏常数(Km)值的影响。结果表明:在引江济太调水试验期间,各湖区水体中vmax及Km值时空分布存在差异性。调水稀释了各湖区蓝藻浓度,抑制了水体碱性磷酸酶vmax;贡湖区、西岸河口区及湖心区水体中Km值与入湖累积水量之间有显著的二次函数关系(p=0.007)。梅梁湖、竺山湖、贡湖及西岸河口区水体碱性磷酸酶vmax/Km随着总磷浓度升高而增加,但竺山湖、贡湖、草型湖、湖心及西岸河口区水体vmax/K随生物可利用磷(PO3--P)浓度增加而下降。除草型湖区外,其它湖区水体v/K随叶绿素a浓度升高而增加。In order to study the influence of water diversion from Yangtze River to Taihu Lake,water samples were collected from Taihu Lake subzones,and the activity of alkaline phosphatase was determined together with water quality parameters.The study is focuses attention on the variation of the maximum reaction rate in alkaline phosphatase activity（vmax） and the Michaelis-Menten constant（Km） due to the water transferring activity.Results show that differences exist in the temporal and spatial distribution of both vmax and Km of Taihu Lake subzones.The concentration of blue-green algae in Taihu Lake subzones is diluted as a result of water diversion,which inhibits the maximum reaction rate in alkaline phosphatase activity.A quadratic relationship（p=0.007） between Km and accumulated water into Taihu Lake is found for the subzones of Gonghu Bay,Western Estuary,and Taihu Lake Centre.The alkaline phosphatase（vmax/Km） increases with the increase of the total phosphorus concentration in the subzones of Meiliang Bay,Zhushan Bay,Gonghu Bay,and Western Estuary.However,vmax/Km decreases with the increase of the bioavailable phosphorus concentration in the subzones of Zhushan Bay,Gonghu Bay,grass-type area,Western Estuary,and Taihu Lake Centre.The vmax/Km is enhanced when the concentration of chlorophylla increases except for grass-type area.

关 键 词：太湖 调水 碱性磷酸酶 最大反应速率vmax 米氏常数Km 

分 类 号：P342[天文地球—水文科学]