双重RT-PCR同时检测兰花两种主要病毒的研究  

Study of dual RT-PCR technique for simultaneous detection two orchid viruses on orchids

作　　者：张妙彬[1] 潘丽晶[1] 肖杨[1,2] 杨玉环[1] 

机构地区：[1]珠海市农业科学研究中心,广东珠海519070 [2]中山大学有害生物控制与资源利用国家重点实验室,广东广州510275

出　　处：《广东农业科学》2010年第5期166-167,180,共3页Guangdong Agricultural Sciences

基　　金：广东省科技计划项目(2006B20130004)

摘　　要：以利用Trizol试剂盒提取的蝴蝶兰组培苗叶片总RNA为模板,根据已报道的建兰花叶病毒(Cymbidium mosaic virus,CyMV)和齿兰环斑病毒(Odontoglussm ringspot virus,ORSV)外壳蛋白基因(cp)的保守序列设计特异性引物,探索RT-PCR反应条件,建立了可同时检测CyMV和ORSV的双重RT-PCR体系。According to the reported Cymbidium mosaic virus （CyMV） and Odontoglussm ringspot virus （ORSV） coat protein （CP） gene sequences, two pairs of specific PCR primers for cp genes were designed respectively. And a dual RT-PCR method was successfully developed for detecting CyMV and ORSV at the same time with the template of total RNA isolated from the leaves of tissue culture plantlets by Trizol reagent kit. The dual RT-PCR method was confirmed as reliable and repeatable by applying to detect the two orchid viruses of several orchid species.

关 键 词：兰花 双重RT-PCR 建兰花叶病毒 齿兰环斑病毒 检测 

分 类 号：S682.31[农业科学—观赏园艺]