人胚胎骨髓基质细胞的体外培养观察  

OBSERVATION ON STROMAL CELL OF HUMAN FETAL BONE MARROW BY CUILTURE IN VITRO

作　　者：苏瑞军[1] 黄绍良[2] 周敦华[2] 李树浓[3] 梁晓燕[1] 

机构地区：[1]广州医学院第一附属医院儿科,510120 [2]中山医科大学逸仙纪念医院儿科 [3]中山医科大学病理生理教研室

出　　处：《中国小儿血液》1999年第1期14-15,共2页China Child Blood

摘　　要：为了探讨人胚胎骨髓基质细胞的生物学特性，我们分别在体外培养人胚胎骨髓基质细胞（ＦＢＭＳＣｓ）和成人骨髓基质细胞（ＡＢＭＳＣｓ），在光镜下观察它们各自的细胞形态，集落形态和大小。结果表明：ＦＢＭＳＣｓ和ＡＢＭＳＣｓ的ＣＦＵ－Ｆ细胞形态，集落形态，集落数，集落大小均无差异，但从出现小丛，形成集落，集落融合交错成片，到集落完全铺满瓶底，ＦＢＭＳＣｓ均晚于ＡＢＭＳＣｓ。表明ＦＢＭＳＣｓ较ＡＢＭＳＣｓ更原始幼稚，可能具有更好的支持造血功能。To study the biological characteristics of the human fetal bone marrow stromal cells, fetal bone marrow stromal cells (FBMSCs) and adult bone marrow stromal cells (ABMSCs)are cultured in vitro and their cell morphology, colony morphology, colony size are observed. The time of their clusters appearance, colonies formation, colonies confluence andspreading out completely are recorded. It is showed that there are no difference between theFBMSCs and ABMSCs in cell morphology, colony morphology, colony numbers and size ofCFU-F. The time of clusters appearance, colonies formation, colonies confluence, spreadingout completely of the FBMSCs is later than that of the ABMSCs. Our studies indicate thatFBMSCs is more primitive than ABMSCs and may have the better functions of supportinghaematopoiesis.

关 键 词：胚胎 骨髓基质细胞 成人 体外培养 

分 类 号：R329－33[医药卫生—人体解剖和组织胚胎学]