尤斯灌流系统在动物胃肠道屏障及营养物质转运中的应用  

Applications of Ussing Chamber in Studying Gastrointestinal Barrier and Nutrient Transport

作　　者：孙志洪[1,2] 贺志雄[1,2] 张庆丽[1,3] 谭支良[1] 

机构地区：[1]中国科学院亚热带农业生态研究所,亚热带农业生态过程重点实验室,长沙410125 [2]中国科学院研究生院,北京100049 [3]西北农林科技大学动物科技学院,杨凌712100

出　　处：《动物营养学报》2010年第3期511-518,共8页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家自然科学基金(30600436);中国科学院知识创新工程重要方向项目(KSCX2-YW-N-051)

摘　　要：本文简要介绍了尤斯灌流(Ussing chamber)系统在动物胃肠道屏障及营养物质转运中的应用,同时提出了在使用Ussing chamber过程中发现的一些问题。建议在应用Ussing chamber系统过程中应使离体研究环境尽量符合胃肠道生理特征,使研究结果更为准确并且更具生理意义,同时通过研究方法的改进使研究材料成本降低。This article introduced the basal components and structures of Ussing chamber and its applications in the field of gastrointestinal barrier function and nutrient transport research. During the process of applying Ussing chamber system,some problems had been found and the solutions had been proposed. It was suggested that in vitro environment needed to be accorded with gastrointestinal physiological characteristics for accurate experimental results and meaningful physiological simulation,simultaneously,material cost was decreased by improvement of methods.

关 键 词：尤斯灌流 胃肠道屏障 物质转运 

分 类 号：S823[农业科学—畜牧学]