泵抑制剂对诱导产ESBLs鸡大肠杆菌抗菌药物敏感性的影响  

Effect of Efflux Pump Inhibitor on Sensitivity of Induced Positive ESBLs Escherichia coli from Chickens to Antibacterial Drugs

作　　者：律海峡[1] 胡功政[1] 苑丽[1] 刘建华[1] 潘玉善[1] 陈玉霞[1] 许瑞[1] 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002

出　　处：《江西农业学报》2010年第5期141-144,共4页Acta Agriculturae Jiangxi

摘　　要：目的:探讨头孢曲松、头孢噻肟、头孢噻呋诱导鸡源大肠杆菌耐药产ESBLs与主动外排机制的关系。方法:用头孢曲松、头孢噻肟、头孢噻呋诱导3株临床分离菌和标准菌O78至产ESBLs;采用琼脂二倍稀释法观察外排泵抑制剂利血平和CCCP对诱导菌抗菌药物敏感性的影响。结果:加入利血平后,头孢曲松诱导后的菌株,阿米卡星、氟苯尼考、磷霉素组的MIC值变化有统计学差异,耐药率下降范围为25%～75%;头孢噻肟诱导后的菌株,头孢曲松、头孢噻呋、多西环素、氟苯尼考、磷霉素组MIC值变化有统计学差异,耐药率下降为25%～100%;头孢噻呋诱导后的菌株,其中头孢曲松、头孢噻呋、阿米卡星、氟苯尼考、磷霉素有统计学差异,耐药率下降为25%～100%。加入CCCP以后,仅头孢噻肟诱导后的菌株,头孢曲松MIC值变化差异显著;头孢噻呋诱导的菌株,头孢曲松、氟苯尼考差异显著;且耐药率下降范围均为25%～75%;利血平与抗菌药物合用后对诱导菌抗菌药物敏感性的影响高于CCCP。结论:3种头孢类药物诱导后的菌株对10种抗菌药物的外排表型存在差异;鸡大肠杆菌特异性耐药机制ESBLs可激活非特异性耐药机制外排表型的过度表达。Objective： To investigate the role of ceftriaxone,cefotaxime and ceftiofur in inducing Escherichia coli to produce ESBLs,and the mechanism of efflux pump inhibitor in positive ESBLs Escherichia coli.Methods： Using different concentrations of ceftriaxone,cefotaxime and ceftiofur to induce 3 clinical isolates of Escherichia coli and O78 to exhibit multidrug-resistant phenotypes in vitro.After inducement,they showed positive ESBLs.Then the effects of efflux pump on the sensitivity of the induced bacteria to ten kinds of antibacterial drugs were measured by agar 2 times-dilution method.Results： After being treated with efflux pump inhibitor reserpine,the MICs of amikacin,florfenicol and fosfomycin had statistical difference,and the decline range of antimicrobial resistance was from 25% to 75% in ceftriaxone-induced bacteria;the MICs of ceftriaxone,ceftiofur,doxycycline,florfenicol and fosfomycin had statistical difference,and the decline range of antimicrobial resistance was from 25% to 100% in cefotaxime-induced bacteria;the MICs of ceftriaxone,ceftiofur,amikacin,florfenicol and fosfomycin had statistical difference,and the decline range of antimicrobial resistance was from 25% to 100% in ceftiofur-induced bacteria.After adding CCCP,the MICs of ceftriaxone had statistical difference in cefotaxime-induced bacteria;the MICs of ceftriaxone and florfenicol had statistical difference in cefotaxime-induced bacteria,the decline range of two antimicrobial resistance was from 25% to 75%.Conclusion： The effects of efflux pump on the sensitivity of three-cephalosporin-drug-induced strains against ten kinds of antibacterial drugs were different,the role of reserpine was higher than that of CCCP,ESBLs could activate the efflux pump.

关 键 词：大肠杆菌 抗菌药物 主动外排泵 泵抑制剂 ESBLS 

分 类 号：S855.12[农业科学—临床兽医学]