柑桔抗羟脯氨酸细胞变异系的选择及其抗寒性研究  

A study on selection of hydroxyproline resistant somaclonal cell line from Citrus and evaluation of its cold tolerance Journal of Zhejiang Agricultural University

作　　者：林定波 颜秋生 沈德绪[1,2,3] 

机构地区：[1]华中农业大学作物遗传改良国家重点实验室 [2]中国水稻研究所 [3]浙江大学园艺系

出　　处：《浙江农业大学学报》1999年第1期94-98,共5页

摘　　要：采用γ射线诱变与高浓度羟脯氨酸（ＨＹＰ）离体选择的方法，获得抗羟脯氨酸（ＨＹＰ）锦橙悬浮细胞系，并再生植株。抗性细胞系比供体细胞系（对照）含有较高的脯氨酸（Ｐｒｏ），有较高的ＰＯＤ与ＣＡＴ活性及较强的抗ＨＹＰ能力，经冷驯化后抗性细胞系的抗寒性比供体细胞提高１．４℃，同时进一步加大了抗性细胞系在抗氧化酶ＳＯＤ、ＣＡＴ活性和脯氨酸（Ｐｒｏ）、可溶性糖含量及水势上与供体的差异。这表明，抗氧化酶活性的提高及渗透调节能力的加强与抗性系抗寒性的增强有关。Somaclonal tolerant cell line and its regenerants were obtained from suspension culture cell of ovules in sweet orange Jincheng ( Citrus sinensis Osb ) via gamma ray mutagenesis and high pressure selection of hydroxyproline (HYP), which were 1 4 ℃ more cold tolerant than the wild types of suspension cells after cold acclimation Besides proline accumulation , soluble sugars content and antioxygenases SOD and CAT activities were stimulated much higher and water potential became much lower than the wild type after cold acclimation at the suspension cell levels, which indicated that not only proline metabolism, but antioxygenases and osmotic regulation were also involved in its enhancement process on cold tolerance

关 键 词：柑桔 抗羟脯氨酸 细胞变异系 抗寒性 渗透调节 

分 类 号：S666.01[农业科学—果树学]