鸡源类人乙肝病毒与人乙肝病毒S基因序列分析  

S Gene Sequence Comparison between the HBV like Virus from Chicken and Human HBV

作　　者：丁壮[1] 金宁一[1] 陈振文[1] 邹啸环[1] 欧阳红生[1] 

机构地区：[1]解放军农牧大学

出　　处：《中国兽医学报》1999年第1期18-21,共4页Chinese Journal of Veterinary Science

摘　　要：为揭示动物乙肝病毒与人乙肝病毒之间的相关性研究打下基础，以人乙肝病毒Ｓ基因两侧的保守区域作为模板，采用套式ＰＣＲ方法，成功地扩增出鸡源类人乙肝病毒部分基因（１．２ｋｂ）。序列分析结果表明，鸡源类人乙肝病毒与人乙肝病毒Ｓ基因似有一定的同源性，但在保守区域（Ｓ区）内存在较大的核苷酸序列差异。To explore the relationship between human hepatitis B virus(HBV) and (HBV) like virus from chicken, S gene fragment (1.2 kb) of hepatitis B virus like virus from chicken was amplified from template of HBV S gene that lies in conservative district with a nested polymerase chain reaction (PCR) successfully. The result of sequence analysis showed that the S gene of HBV like virus from chicken harbors some sequences homologous with HBV S gene, but there are different nucleotide sequences in conservative district (S district).This study provides a basis for researching the relationship between the HBV like virus and the HBV.

关 键 词：类人乙肝病毒 S基因 序列分析 鸡 

分 类 号：S858.312.6[农业科学—临床兽医学]