猪2-细胞胚胎细胞的电融合  

Electrofusion of Porcine Two cell Embryonic Cells

作　　者：陈乃清[1] 赵浩斌[1] 苟德明[1] 樊俊华[1] 魏庆信 

机构地区：[1]武汉大学生命科学学院

出　　处：《中国兽医学报》1999年第1期89-91,共3页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金

摘　　要：为了探索猪胚胎细胞核移植技术中细胞融合参数，进行了猪２－细胞胚胎细胞的电融合。电融合时，直流电场强度为２ｋＶ／ｃｍ，脉冲时程选４０μｓ时，可获得７０．４％的融合率和６８．４％的发育率。非电解质融合液（甘露醇）对于融合率和融合胚的发育均优于电解质融合液（ＤＰＢＳ）。在直流电脉冲前的交流脉冲是必要的，可以显著提高融合率（Ｐ＜０．０５）。The techniques on electrofusion of porcine two cell embryonic cells were systematically studied. When direct current field strength was 2 kV/cm and pulse duration was 40 μs, the fusion rate and developmental rate of the fused embryo accounted for 70.4% and 66.7%, respectively. Comparison of nonelectrolyte and electrolyte electrofusion media showed that the nonelectrolyte was better than electrolyte for fusion rate and development rate. In addition, it has been confirmed that the alternating current pulse antedated direct current should be necessary for the embryonic cells to be fused, with the significantly increased fusion rate (P<0.05).

关 键 词：电融合 猪 胚胎细胞融合 

分 类 号：S828.3[农业科学—畜牧学]