弗兰克氏菌Cel 517菌株固氮研究  

Studies on Nitrogen Fixation of Frankia sp Cel 517 Strain

作　　者：郝家骐 朱世琴[1] 周鸿宾 魏玉芬 苏小芳[1] 

机构地区：[1]山西省生物研究所

出　　处：《山西农业科学》1999年第1期57-59,共3页Journal of Shanxi Agricultural Sciences

摘　　要：由木麻黄（ＣａｓｕａｒｉｎａｅｇｕｉｓｅｔｉｆｏｌｉａＬ）根瘤中分离的内生菌ＦｒａｎｋｉａｓｐＣｅｌ５１７菌株，可在以丙酸为碳源的限定培养基中表现出较高的固氮酶活性。固氮周期在１８０ｈ达到高峰。蛋白胨与ＮＨ４Ｃｌ对固氮活性有抑制作用，氧分压为１０％～１５％时，固氮酶活性最高，泡囊的诱导时间对固氮酶活性影响较大。Frankia sp Cel 517 can show higher nitrogenase activity in a defined medium containing propionic acid as C_source. The maximal nitrogenase activity of Cel 517 arrived at 180 hours. Proteinase and NH 4Cl inhibited nitrogenase activity. The highest nitrogenase activity was obtained in 10%～15% of O 2. The time of induced vesicles has great influence on nitrogenase.

关 键 词：FRANKIA 固氮 弗兰克氏菌 Cel517菌株 

分 类 号：Q939.113[生物学—微生物学] Q945.13