甘草提取物的体外抗氧化活性  

Antioxidant activity of licorice extracts in vitro

作　　者：张改平[1] 朱华泽[2] 杨建雄[3] 

机构地区：[1]空军工程大学理学院,西安710051 [2]西安科技大学理学院,西安710054 [3]陕西师范大学生命科学院,西安710062

出　　处：《生物加工过程》2010年第3期53-57,共5页Chinese Journal of Bioprocess Engineering

基　　金：国家自然科学基金资助项目(20175012)

摘　　要：为研究甘草提取物的体外抗氧化作用,采用60%乙醇回流提取甘草(LF),甘草粗提物(LF0)通过AB-8吸附树脂纯化制取甘草精提物(LF1),对LF0和LF1主要活性成分的含量进行测定。分别采用DPPH法、2-脱氧-D-核糖法和NBT-NADH-PMS体系、β-胡萝卜素-亚油酸体系测定LF0和LF1对1,1-二苯基-2-苦苯肼自由基(DPPH.)、羟自由基(OH.)、超氧阴离子自由基(.O-2)的清除能力以及LF0和LF1的总抗氧化能力;通过对LF0和LF1还原能力的测定分析LF0和LF1的抗氧化活性。研究结果显示,LF0和LF1能有效清除.O2-、DPPH.和OH.,具有良好的总抗氧化能力和还原能力,且LF1的作用比LF0好。Licorice flavonoid（LF） was extracted from licorice with 60% aqueous ethanol by refluxing.The crude LF（designated as LF0） was purified by absorbent resin AB-8 to obtain LF1.Contents of the active components in LF0 and LF1 were measured.The scavenging activity on DPPH,OH· and ·O-2radicals in LF0 and LF1 was evaluated by DPPH method,2-deoxy-D-ribose method,NBT-NADH-PMS system,and β-carotene linoleate model system,respectively.The total antioxidant activity of LF0 and LF1 was assayed by measuring the reducing power.The investigation showed that LF0 and LF1 had strong radical scavenging activity towards DPPH·,OH· and ·O-2,and both of them had good total antioxidant activity and reducing power.The activity of LF1 was stronger than that of LF0.

关 键 词：甘草提取物 抗氧化 自由基 清除作用 

分 类 号：R284[医药卫生—中药学]