人胎盘中胶原蛋白的提取和表征  

Extraction and characterization of human-placenta collagen

作　　者：李德荣[1] 于相梅 朱斌[2] 

机构地区：[1]临沂市兰山区人民医院,临沂276000 [2]四川职业技术学院,遂宁629000

出　　处：《生物加工过程》2010年第3期58-61,共4页Chinese Journal of Bioprocess Engineering

基　　金：四川省教育厅自然科学科研基金资助项目(072C042)

摘　　要：采用碱-酶结合法以人胎盘为原料提取胶原蛋白,并使用原子力显微镜(AFM)、差示扫描量热仪(DSC)、圆二色光谱(CD)和傅立叶变换红外光谱(FTIR)对所得胶原蛋白产品进行表征。结果表明:此工艺路线的胶原蛋白得率高,达到0.102%;提取时间由48 h以上减少到24 h以内,而且所得胶原蛋白分子完整地保留了其螺旋纤维结构。此法是从人胎盘中提取胶原蛋白的一种行之有效的方法。The collagen was extracted from the human-placenta by a alkali-enzyme method.The properties of the collagen were investigated by atomic force microscope（AFM） and differential scanning calorimeter（DSC）,circular dichroism（CD）,and Fourier transform infrared spectroscopy（FTIR）.The results indicated that the collagen yield was 0.102% by the new method.The extraction time was reduced from 48 h to 24 h.In addition,the collagen molecules held the fibrillar structure.The new method was effective for extracting the collagen from human-placenta.

关 键 词：表征 人胶原蛋白 提取 胎盘 

分 类 号：Q819[生物学—生物工程]