丹参酮Ⅱ_A对阿霉素抑制肿瘤细胞生长作用的影响  

Effects of TanshinoneⅡ_A on Aadriamycin-induced suppression of tumor cells growth

作　　者：高洁[1,2] 白茹[1] 李长志[1] 刘培庆[2] 

机构地区：[1]南开大学药学院,天津300071 [2]中山大学药学院,广东广州510080

出　　处：《药物评价研究》2010年第2期91-94,共4页Drug Evaluation Research

摘　　要：目的:观察丹参酮ⅡA对阿霉素诱导2种肿瘤细胞(K562和Bel-7402细胞)死亡作用的影响。方法:MTT法检测细胞存活率,Hoechst33342染色从形态学上反映肿瘤细胞凋亡情况。结果:丹参酮ⅡA本身对2种肿瘤细胞均有抑制增殖、促凋亡的作用,不会干扰阿霉素诱导肿瘤细胞死亡的作用,甚至对其对K562细胞的促凋亡作用有协同效果。结论:丹参酮ⅡA对阿霉素抑制肿瘤细胞生长有协同作用,但对肿瘤细胞有一定的选择性。Objective： To investigate the effects of Tanshinone ⅡA on Adriamycin-induced suppression of growth in two kinds of tumor cell （K562 and Bel-7402）. Methods： MTT colorimetric assay was used to detect the survival rate of tumor cells. Hoechst 33342 staining was used to reflect tumor cells apoptosis morphologically after different treatments. Results： Tanshinone IIA alone induced cell death in K562 cells and human hepatocarcinoma Bel-7402 cells. It did not interfere with the antiproliferative effects of Adriamycin in these two cells. When used with Adriamycin, Tanshinone ⅡA even enhanced cell death induced by Adriamycin in K562 cells. Conclusion： Tanshinone ⅡA showes synergistic effect with Adriamycin in suppression of tumor cells growth, however, with cell type specificity.

关 键 词：丹参酮ⅡA 阿霉素 肿瘤细胞 

分 类 号：R979.1[医药卫生—药品]