石崖茶水溶性多糖的提取及生物活性研究  

Study on the Extraction and Biological Activities of Water Soluble Polysaccharide from Adinandra Nitride

作　　者：刘春兰[1] 杨霞[1] 白冬梅[1] 李阳[1] 肖欢涢[1] 

机构地区：[1]中央民族大学生命与环境科学学院,北京100081

出　　处：《辽宁中医药大学学报》2010年第6期10-12,共3页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：高等学校学科创新引智计划(B08044);中央民族大学2008年度无机化学优秀课程建设项目;中央民族大学URPT项目(200910059)

摘　　要：石崖茶经热水浸提,乙醇沉淀得粗多糖(SC)。粗多糖经凯氏定氮法测得蛋白质含量为7.38%,苯酚-硫酸法测定多糖含量为57.36%,硫酸-咔唑法测糖醛酸含量为24.11%。粗多糖经Sevage法和酶法联合脱蛋白,得初步纯化的多糖(SC1)。气相色谱分析其单糖组成为:鼠李糖(Rha)、阿拉伯糖(Ara)、半乳糖(Gal)、葡萄糖(Glc)。摩尔比为:0.1:0.28:6.3:1。红外光谱分析,SC1具有多糖的典型性质。石崖茶多糖对羟基自由基和超氧自由基具有清除能力,且纯化后多糖的清除效果比粗多糖好。The crude polysaccharide SC was extracted from Adinandra nitida Merr.exLi with hot water,and precipitated by ethanol.Then some identifiable properties of the crude polysaccharide（SC）were studied.It contained of no starch.The contents of protein was 7.38%,the contents of GAL-A was 24.11% on phenovitriol method and the contents of Adinandra nitida Merr.exLi polysaccharide was 57.36%.The refined polysaccharide（SC1）was prepared after protein being separated.GC analysis indicated that SC1 was composed of Rah,Ara,Gal,Glc and its molar ratio is ：0.1：0.28：6.3 ：1.IR showed that SC1 had a character of polysaccharide.Both SC and SC1 were effective in scavenging super oxide radical and hydroxyl radical.The scavenging capacity for super oxide radical and hydroxyl radical of SC1 had higher than that of SC.

关 键 词：石崖茶 多糖 纯化 清除自由基作用 

分 类 号：R917[医药卫生—药物分析学]