中国不同地区水稻干尖线虫种群的繁殖特性研究  

Reproductive fitness of 15 Aphelenchoides besseyi populations from China

作　　者：裴艳艳[1] 骆爱丽[1] 谢辉[1] 杨再福[2] 程曦[1] 徐春玲[1] 

机构地区：[1]华南农业大学资源环境学院植物线虫研究室,植物检疫线虫检测与防疫研究中心,广东广州510642 [2]贵州大学农学院,贵州贵阳550025

出　　处：《西北农林科技大学学报（自然科学版）》2010年第6期165-170,共6页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家自然科学基金项目(30871629)

摘　　要：【目的】明确来自中国不同地区的水稻干尖线虫种群的繁殖特性。【方法】通过室内组织培养的方法,研究来自中国8个省15个种群的水稻干尖线虫,在灰葡萄孢菌上的繁殖力及其繁殖适温。【结果】在灰葡萄孢菌上,供试的15个水稻干尖线虫种群在20℃不能很好繁殖,其中有9个种群的繁殖适温是25℃,6个种群的繁殖适温是30℃;20℃时,繁殖量最大种群的繁殖率是22.2,繁殖量最小种群的繁殖率仅有0.9,有3个种群无雄虫存在;25℃时,繁殖量最大种群的繁殖率是3 523.8,繁殖量最小种群的繁殖率是207.2,只有1个种群无雄虫存在;30℃时,繁殖量最大种群的繁殖率是1 525.0,繁殖量最小种群的繁殖率是6.9,所有种群均有雄虫存在。【结论】来自中国不同地区的水稻干尖线虫种群均能在灰葡萄孢菌上繁殖,种群之间的繁殖力和繁殖适温均存在差异,地理来源较远的种群之间差异较大;在适温条件下,华南地区多数种群的繁殖力大于其他地区,种群繁殖特性与地理来源有一定关系;所有种群均能孤雌生殖,后代均以雌虫为主,但不同种群的雌雄比有较大差异。【Objective】 The reproduction of 15 Aphelenchoides besseyi populations from 8 provinces of China was tested in this study.【Method】 The culture of A.besseyi was on Botrytis cinerea Pers 35 days after inoculation with 20 females at 20,25 and 30 ℃ in dark.【Result】 The temperature 20 ℃ was unfavorable for the reproduction of the 15 populations,25 ℃ was the optimum temperature for the reproduction of 9 populations,and 30 ℃ for the reproduction of the other 6 populations.At 20 ℃,the highest reproductive rate was 22.2,the lowest 0.9,and 3 populations had no males.At 25 ℃,the highest reproductive rate was 3 523.8,the lowest 207.2,and only 1 population had no males.At 30 ℃,the highest reproductive rate was 1 525.0,the lowest 6.9,and all populations had males.【Conclusion】 The results demonstrated that 15 A.besseyi populations can reproduce on B.cinerea Pers,there were significant differences on reproduction and optimum temperature of A.besseyi populations.At optimum temperature,the reproduction of A.besseyi populations form South China was higher than that of others.It indicated that there were some relativities between the reproduction of A.besseyi and their geographical origins.All tested populations can reproduce by parthenogenesis,there were more females in their offspring and the sex ratio was significantly different between populations.

关 键 词：水稻干尖线虫 繁殖力 灰葡萄孢菌 温度 

分 类 号：S435.111.48[农业科学—农业昆虫与害虫防治]