油菜花粉提取物对前列腺癌细胞DU-145的体外生长抑制研究  

Inhibitory effect of Brassica campestris pollen extract on prostate cancer cell line DU-145 growth in vitro

作　　者：顾琳慧[1] 钱丽娟[1] 杜灵彬[1] 姜志明[1] 高云[1] 凌雨田[1] 曹明富[2] 

机构地区：[1]浙江省肿瘤医院研究所,杭州310022 [2]杭州师范大学生命与环境科学学院,310036

出　　处：《中华危重症医学杂志（电子版）》2010年第1期9-12,共4页Chinese Journal of Critical Care Medicine:Electronic Edition

基　　金：浙江省科技攻关计划项目(2005C3305)

摘　　要：目的探讨油菜花粉脂溶性提取物(BPE)对体外培养的人前列腺癌细胞DU-145的生长抑制及作用机制。方法采用不同浓度的BPE作用于体外生长的人前列腺癌细胞DU-145。通过CCK-8法检测BPE对DU-145的增殖抑制,透射电镜观察细胞形态,流式细胞术测定细胞周期的改变,RT-PCR测定促凋亡基因Bax和抑凋亡基因Bcl-2的表达。结果 BPE对DU-145细胞具有增殖抑制作用,且在一定范围内具有剂量依赖性。BPE浓度为1000μg/ml时,DU-145细胞抑制率以48h尤为显著,达到79.4%,与对照组比较,有显著性差异(P<0.001)。低、中浓度的BPE作用24h后,对细胞生长抑制不明显。BPE可以引起细胞凋亡,同时改变细胞周期。随着浓度的升高,DU-145细胞G0/G1期比例明显增加,S期比例下降。BPE对细胞凋亡的影响显示阴性时,Bax表达较低,低浓度BPE使其表达大量增加;随着药物作用浓度增加,Bax表达反而降低。阴性时,Bcl-2表达较低,低浓度BPE使其表达大量增加;随着药物作用浓度增加,Bcl-2表达急剧降低。Bax/Bcl-2比值在阴性时最高,随着药物浓度升高而降低。结论 BPE对人前列腺癌细胞DU-145的体外生长具有抑制效应。Objective To investigate the inhibitory effect of Brassica campestris pollen extract （BPE） on growth of human prostate cancer cell line DU-145 in vitro. Methods BPE with different concentrations was added into DU-145 cells. CCK-8 assay was used to detect the inhibitory effect of BPE on DU-145. Transmission electron microscope and flow cytometry were adopted to observe and determine cell shapes and cycle changes. Moreover,RT-PCR was applied to assess the expression of promoting apoptosis gene （Bax） and antiapoptosis gene （Bcl-2）. Results The growthinhabitation rate of BPE on DU-145 cells increased in dose-dependent manner,and approached to climax （79.4% ） at 48 h when concentration of BPE reached 1000 μg/ml,which showed significant difference,compared with the control group （P0.001）. Low and moderate concentration of BPE had little effect on the proliferation of DU-145 cells after 24 h. BPE could reduce the apoptosis,meanwhile,change the cell cycle. With the increase of concentration,the ratio of DU-145 cells in G0/G1 phase were obviously elevated,while in S phase decreased. The expression of Bax and Bcl-2 were low when the effect of BPE on apoptosis showed negative. Low concentration of BPE could increase their expression. However,the higher concentration of BPE,the lower expression of Bax and Bcl-2 were. The ratio of Bax/Bcl-2 reached the peak when the effect was negative,and declined when the concentration increased. Conclusion BPE can inhibit the growth of prostate cancer cell line DU-145 in vitro.

关 键 词：油菜花粉提取物 前列腺癌 细胞凋亡 细胞周期 

分 类 号：R285[医药卫生—中药学]