胀果甘草查尔酮合成酶基因cDNA的克隆及序列分析  

Molecular Cloning and Analysis of Chalcone Synthase Gene cDNA in Glycyrrhiza inflata

作　　者：梁玉玲[1] 姚红宝[1] 曲占良[1] 许恒飞[1] 

机构地区：[1]河北大学生命科学学院,河北保定071002

出　　处：《河北大学学报（自然科学版）》2010年第3期313-318,共6页Journal of Hebei University(Natural Science Edition)

基　　金：河北省教育厅基金资助项目(2007411);河北省生物工程重点学科资助项目

摘　　要：为进一步利用基因工程手段调控甘草黄酮的合成,通过反转录-聚合酶链式反应(RT-PCR)方法,从胀果甘草愈伤组织中克隆查尔酮合成酶(chalcone synthase,CHS)基因的cDNA,运用DNAMAN软件对序列进行分析,运用PHYLIP 3.67软件绘制系统进化树.克隆得到的基因片段全长为1 170 bp,包含1个完整的开放阅读框架(ORF),编码1个由389个氨基酸残基组成的多肽.该基因片段与紫花苜蓿、大豆、豌豆等几种豆科植物的CHS核苷酸同源率高达80%以上,氨基酸同源率高达90%以上.表明克隆得到了胀果甘草chs基因cDNA,序列提交GenBank注册,序列号为EU706287.The chalcone synthase（CHS） gene cDNA of Glycyrrhiza inflate was cloned from total cDNA of licorice callus via RT-PCR;The cDNA sequence was analyzed through DNAMAN software;The phylogeneitc tree based on the amino acid sequences of CHS was built by PHYLIP 3.67 software.A cDNA fragment of 1 170 bp length was obtained,the fragment contains a complete open reading frame（ORF） encoding 389 amino acids,it has more than 80% sequence homology at the nucleotide level and more than 90% similarity at the amino acid level compared with Medicago sativa,Glycine max,Pisum sativum etc.The chs gene cDNA of G.inflate was sullessfully obtained,the sequence was submitted to the GenBank,the accession number is EU706287.

关 键 词：胀果甘草 反转录-聚合酶链式反应(RT-PCR) 查尔酮合成酶 CDNA 

分 类 号：Q945.5[生物学—植物学]