新人参二醇血浆样品制备方法初探  

Preliminary Studies on the Preparation Method of NPD plasma samples

作　　者：赵俊艳[1] 刘继华[2] 朱枫[1] 孟勤[2] 尹建元[2] 刘莹[2] 倪加勉[2] 

机构地区：[1]吉林大学白求恩制药厂,吉林长春130021 [2]吉林大学药学院,吉林长春130021

出　　处：《特产研究》2010年第2期46-48,61,共4页Special Wild Economic Animal and Plant Research

基　　金：长春市科技计划项目(08YJ41)

摘　　要：建立新人参二醇血浆样品制备方法。以高效液相色谱法检测小鼠血浆提取物中新人参二醇的含量,确定小鼠血浆预处理方法。结果石油醚、乙醚和醋酸乙酯等萃取法制备的血浆样品中新人参二醇提取率低;甲醇萃取法、SPEC18固相萃取法制备的血浆样品提取率均较高,但甲醇萃取制备的样品中杂质峰干扰较大,而SPEC18固相萃取法制备的血浆样品中杂质峰对样品无干扰。SPEC18固相萃取法可作为新人参二醇血浆样品的制备方法。Objective To establish the preparation method of extracting New Panaxadiol（NPD）from Rice plasma samples.Methods The content of NPD in the Rice plasma was determined by high-performance liquid chromatography,and the pre-process method of the Rice plasma was obtained.Results：The extraction rates by petroleum ether,ethyl ether and ethyl acetate were lower than by methanol or by solid-phase extraction method.The impurity had interference with NPD in methanol extracts,but impurity had no interference with NPD prepared by solid-phase method.Conclusion：Solid-phase method with SPEC18 is the most suitable for preparing the NPD plasma samples.

关 键 词：新人参二醇 血浆样品 溶剂萃取 固相萃取 

分 类 号：S567.51[农业科学—中草药栽培] O658.2[农业科学—作物学]