2型糖尿病大鼠肺组织基质金属蛋白酶-2、9及其抑制因子-1的表达变化  

Changes of MMP-2,MMP-9,TIMP-1 in lung tissue of type 2 diabetic rats

作　　者：穆维新[1] 沈亚欣[2] 崔士杰[2] 王绵[3] 

机构地区：[1]河北医科大学第四医院内科,石家庄市050011 [2]河北医科大学外科总论教研室 [3]河北医科大学第二医院内分泌科

出　　处：《河北医药》2010年第9期1034-1037,共4页Hebei Medical Journal

基　　金：河北省医学科学研究重点课题(编号:07057)

摘　　要：目的通过检测2型糖尿病大鼠肺组织中基质金属蛋白酶-2(MMP-2)、基质金属蛋白酶-9(MMP-9)及基质金属蛋白酶抑制因子-1(TIMP-1)的表达变化,探讨MMP-2、MMP-9及TIMP-1在糖尿病肺损伤中的作用。方法通过高糖、高脂饮食加腹腔注射小剂量链脲佐菌素(streptozotocin,STZ)的方法 ,建立2型糖尿病大鼠模型,采用光镜动态观察12周、24周时对照组、糖尿病组的大鼠肺组织的形态学改变,用Masson三色染色观察肺组织胶原沉积情况,应用免疫组织化学方法检测2组大鼠肺组织MMP-2、MMP-9及TIMP-1的动态表达变化情况。结果糖尿病大鼠肺组织胶原含量明显增多,糖尿病大鼠肺组织MMP-2、MMP-9、TIMP-1表达增多,同时MMP-2/TIMP-1、MMP-9/TIMP-1比例也增加。随病情进展,上述变化更加明显。结论糖尿病大鼠出现了肺间质纤维化,MMP-2、9及TIMP-1表达增多及其比例紊乱参与了糖尿病肺组织病变的发生发展,可能是糖尿病肺纤维化发生的机制之一。Objective To investigate the changes of MMP-2, MMP-9, and TIMP-1 in lung tissue of diabetic rats,and to explore the roles of MMP-2, MMP-9, TIMP-1 in lung injury of diabetic rats. Methods The experimental models of type 2 diabetic rats were established by injecting streptozotocin （STZ） and feeding high fat and glucose food, then the changes of morphology in the lung of rats were observed by light microscope at 12 weeks and 24 weeks respectively, and the collagen accumulation of lung was observed by masson trichrome staining , and the levels of MMP-2, MMP-9 and TIMP-1 in lung were detected by immunohistochemistry. Results The amount of collagen fibre was increased obviously in lung tissue of diabetic rats, the expression of MMP-2, MMP-9, TIMP-1 and the ratio of MMP-2/TIMP-1, MMP-9/TIMP-1 was also increased in lung tissue of diabetic rats. With the progression of pathological changes, the changes became much more obvious. Conclusion There is pulmonary fibrosis in diabetic rats. The changes of MMP-2, MMP-9, TIMP-1 and the ratio of MMP-2/TIMP-1, MMP-9/ TIMP-1 in lung tissue of dibtetic rats may be related to lung tissue pathological changes ,which may be one of the mechanisms for diabetic lung fibrosis.

关 键 词：糖尿病 肺纤维化 基质金属蛋白酶-2 基质金属白酶-9 基质金属蛋白酶抑制物-1 

分 类 号：R587.1[医药卫生—内分泌]