培养基成分对葡萄愈伤组织生长和白藜芦醇含量的影响  

Effects of Medium Concentration on Callus Growth and Resveratrol of Grape

作　　者：邓建平[1] 杨国顺[2] 黄益鸿[1] 李益锋[1] 周杰良[1] 

机构地区：[1]湖南生物机电职业技术学院植物科技系,湖南长沙410427 [2]湖南农业大学园艺园林学院,湖南长沙410128

出　　处：《北方园艺》2010年第11期134-137,共4页Northern Horticulture

基　　金：湖南省农业厅资助项目(湘农业计2007(354号))

摘　　要：以巨峰葡萄果皮愈伤组织为试材,利用不同成分的B5继代培养基对其进行诱导,测定愈伤组织生长量及白藜芦醇含量。结果表明:5%蔗糖有利于愈伤组织的生长及白藜芦醇含量的积累;NO3-/NH4+比例过高或过低均不利于愈伤组织的生长及白藜芦醇含量的积累,二者比例为2∶1时效果最佳;添加5 mg/L NAA与0.7 mg/L6-BA有利于愈伤组织的生长及白藜芦醇含量的积累。在最适宜培养基条件下,愈伤组织的生长量为56.12 g/L,白藜芦醇为464.520μg/g,白藜芦醇总含量为23.312 mg/L。白藜芦醇的含量和产量分别是对照的2.6倍和2.9倍。Taking Kyoho grape peer callus for materials, using callus at different subculture medium, callus growth and the resveratrol content were determined. The results showed that 5 % sucrose in medium was favorable for callus growth and the resveratrol content; High or low of NO3-/NH＋ was unfavorable for callus growth and the resveratrol content, the n^aximum were achieved when NO3-/NH4＋ ratio was 2 ： 1;B5 subculture medium added 5 mg/L NAA and 0. 7 mg/L 6-BA was favorable for callus growth and the resveratrol content. In the most suitable medium under the conditions of callus growth was 56. 12 g/L, the yield of resveratrol was 464. 520 μg/g, the total content of resveratrol was 23. 312 mg/L. The resveratrol content and yield of the control were 2.6 times and 2.9 times.

关 键 词：培养基成分 葡萄愈伤组织 白藜芦醇 

分 类 号：S663.1[农业科学—果树学]