人胎盘提取物对脐带血造血干细胞的体外扩增实验研究  

Effect of human placental extract(intacellin) on proliferation of human umbilical cord blood hematopoietic stem cells in vitro

作　　者：刘禄斌[1] 刘畅[1] 张华[1] 刘菊莲[1] 陈凤娴[1] 

机构地区：[1]重庆市妇幼保健院,400013

出　　处：《重庆医学》2010年第11期1342-1343,1346,共3页Chongqing medicine

摘　　要：目的研究人胎盘提取物对脐带血造血干细胞体外扩增的影响。方法分离脐带血造血干细胞体,用常规培养法和加入人胎盘提取物的方法进行体外扩增培养,扩增培养第1、2、3、4周分别进行单个核细胞(MNC)计数和造血祖细胞集落培养及计数。结果到第4周时胎盘提取物组MNC扩增最高可达78.25倍,而常规培养组仅增加了8.32倍;造血祖细胞集落计数显示第3周达高峰,第4周逐渐下降,但胎盘提取物组扩增集落形成细胞(CFC)的能力明显高于常规培养组。结论胎盘提取物能有效促进脐带血造血干细胞的体外增殖,但应避免长时间的体外扩增培养,以防止细胞发生分化,影响扩增质量。Objective To investigate the effect of human placental extract（Intacellin） on proliferation of human umbilical cord blood hematopoietic stem cells in vitro.Methods After hematopoietic stem cells were isolated from umbilical cord blood,the cells were culture in routine method or with human placental extract,and MNC count and colony-forming cell（CFC） culture were performed at week 1,2,3 and 4.Results The number of MNCs increased and reached 78.25-fold in human placental extract culture group,while 8.32-fold in routine culture group at week 4.CFC increased and reached the peak at week 3,the total number of CFC was higher in human placental extract culture group than that in routine culture group,then a rapid decline was observed at week 4.Conclusion Expansion ability of hematopoietic stem cells from cord blood in media with human placental extract is better than that in routine culture system without human placental extract,but long tome culture in vitro should avoid to prevented cell differentiation and remain expansion quality.

关 键 词：人胎盘提取物 脐带血造血干细胞 扩增 

分 类 号：R285.5[医药卫生—中药学]