枇杷叶紫珠中苯乙醇苷类成分研究  

Component Analysis of Phenylethanoid Glycosides from Callicarpa kochiana Makino

作　　者：林朝展[1] 柴玲[1] 蔡昭喜[1] 赵钟祥[1] 张翠仙[1] 祝晨蔯[1] 

机构地区：[1]广州中医药大学中药学院,广中尖峰天然产物共建实验室,广州510006

出　　处：《中药新药与临床药理》2010年第3期276-279,284,共5页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家中医药管理局重大科技专项(国中医药科2001ZDZX005);广东省国际合作项目(2008A050200005)

摘　　要：目的研究枇杷叶紫珠中高极性化学成分,为其药效成分及作用机制的深入研究奠定基础。方法采用甲醇提取,不同极性溶剂分布萃取,获得正丁醇萃取部位;采用大孔树脂、反相硅胶、Sephadex LH-20、Toy-opearl HW-40等色谱技术进行分离纯化,综合运用紫外光谱(UV)、红外光谱(IR)、质谱(MS)及核磁共振光谱(1H NMR、13C NMR、1H-1H COSY、HSQC、HMBC)等现代波谱学方法和技术进行化合物的结构鉴定。结果自正丁醇萃取部位中共分离得到8个化合物,鉴定出其中6个化合物的结构,分别为毛蕊花糖苷acteoside(1),连翘苷forsythosides B(2),肉苁蓉苷C cistanoside C(3),异毛蕊花糖苷isoacteoside(4),肉苁蓉苷D cistanosideD(5)和独一味苷A lamiophlomioside A(6)。结论所鉴定的6个化合物均为首次自该种植物中分离得到,其中肉苁蓉苷C(3)、肉苁蓉苷D(5)和独一味苷A(6)为首次自紫珠属中分离得到。苯乙醇苷类成分为枇杷叶紫珠中高极性部位主要化学成分。Objective To study the high-polarity chemical constituents of Callicarpa kochiana Makino,thus to supply evidence for its pharmacological research and therapeutic mechanism.Methods The chemical constituents of Calli-carpa kochiana Makino were extracted by methanol and different polar solvents to obtain n-butanol extract.Then n-butanol extract was isolated and purified by macroporous resin,reversed phase silica gel,Sephadex LH-20 and Toyopearl HW-40.The structure of n-butanol extract was identified by spectral analysis of ultraviolet spectrum,in-frared spectrum,mass spectrum,and NMR spectrum.Results We obtained 8 chemical compounds from n-butanol extract.Of them,six phenylethanoid glycosides were identified as acteoside（1）,forsythosides B（2）,cistanoside C（3）,isoacteoside（4）,cistanoside D（5）,lamiophlomioside A（6）.Conclusion Compound 3,5 and 6 is isolated from Callicarpa genus plant for the first time,and phenylethanoid glycosides are the main chemical constituents from n-butanol fraction of Callicarpa kochiana Makino.

关 键 词：枇杷叶紫珠 化学成分 苯乙醇苷类 

分 类 号：R284.1[医药卫生—中药学]