冬虫夏草提取液对糖尿病小鼠肝线粒体氧化磷酸化功能保护作用  

PROTECTIVE EFFECT OF EXTRACT OF CORDYCEPS SINENSIS ON OXIDATIVE PHOSPHORYLATION FUNCTION OF LIVER MITOCHONDRIA IN DIABETIC MICE

作　　者：张蕾[1] 陈顺志[1] 刘树森[2] 

机构地区：[1]辽宁省辽宁医学院,锦州121000 [2]中国科学院动物研究所生物膜与膜生物国家重点实验室

出　　处：《现代预防医学》2010年第11期2031-2033,共3页Modern Preventive Medicine

摘　　要：[目的]用四氧嘧啶(Alloxan)建立实验性糖尿病小鼠模型,并同时用冬虫夏草提取液进行保护,探讨冬虫夏草提取液对糖尿病小鼠肝线粒体氧化磷酸化功能的保护作用。[方法]从线粒体水平测定:①以琥珀酸(S)为底物的呼吸控制:态3呼吸速率(R3)、态4呼吸速率(R4)、呼吸控制比(RCR)和磷/氧比(P/O);②H+-ATPase合成活力;③测定O2·-的生成量;④SOD活性及GSH含量。[结果]糖尿病小鼠ATP合酶活性显著降低45.99%;O2·-含量增加35.8%;线粒体呼吸链RCR、P/O显著降低(P﹤0.01),State4显著增高(P﹤0.01);线粒体SOD活性,GSH含量显著降低,P﹤0.001,用虫草提取液进行保护,各项指标均有显著恢复。[结论]证明实验性糖尿病小鼠肝功能损伤是氧化性损伤,氧化应激又是该损伤的结果,其原因与电子漏增加为主,从而导致质子漏增加,自由基生成量增多,ATP合酶活性下降,无效耗氧增加、线粒体受损;同时也证明了虫草提取液能有效的拮抗由糖尿病引起的肝线粒体的氧化损伤。[Objective] Experimental animal diabetic model established with Alloxan and extract of cordyceps sinensis were used to investigate the mechanism for changes of mitochondrial oxidative phosphorylation during the diabetes and the protective function of cordyceps sinensis. [Methods] We determined parameters of mitochondria respiratory： ①control in the presence of Succinate （S）： respiratory rate of state 4 （Rate 4）, respiratory rate of state 3 （Rate 3）, respiratory control rate （RCR） and P/O; ② H＋-ATPase synthesis activity; ③contents of super oxide anion O2·-; ④activity of antioxidant enzyme. [Results] In diabetic mice, H＋-ATPase synthesis activity decreased by 45.99%, the mitochondrial O2·- of liver increased by 35.8%; as well as the decrease in RCR and P/O with both substrates （P﹤0.01）. Rate 4 increased significantly （P﹤0.01）. The significant decreases of SOD activity and GSH contents were observed in liver mitochondria （P﹤0.001）. It found that data recovered remarkably after breeding to diabetes mice with extract of codyceps sinensis. [Conclusion] Liver function damage of diabetic mice is oxidative damage, and oxidative irritability is the result of oxidative damage. The enhanced mitochondrial electron leak induces proton leak, the content of free radicals increases, H＋-ATPase synthesis activity decreases and the ineffective increase of oxygen consumption might be responsible for the function of mitochondrial damage during diabetes; and extract of codyceps sinensis is effective in resisting the oxidative damage on liver mitochondria of diabetes mice.

关 键 词：线粒体 呼吸活性 超氧阴离子 糖尿病 冬虫夏草提取液 

分 类 号：R587.1[医药卫生—内分泌]