亚砷酸钠对L-02肝细胞的氧化损伤作用  

OXIDATIVE INJURY INDUCED BY SODIUM ARSENITE IN L-02 HEPATIC CELLS

作　　者：罗鹏[1] 王赟[1] 张爱华[1] 张开菊[1] 曾小盼[1] 

机构地区：[1]贵阳医学院公共卫生学院卫生毒理学教研室,贵阳550004

出　　处：《现代预防医学》2010年第11期2137-2139,共3页Modern Preventive Medicine

基　　金：贵州省教育厅基金项目(黔教科2006311);贵阳医学院博士基金项目(C2007-13);贵阳医学院研究生教育创新计划专项经费资助项目

摘　　要：[目的]探讨亚砷酸钠(NaAsO2)对体外培养的人肝细胞株(L-02)的氧化损伤作用。[方法]用不同浓度NaAsO2(50,100,150μmol/L)染毒L-02肝细胞24h,MTT法检测NaAsO2对L-02肝细胞生长的影响;流式细胞仪检测L-02肝细胞内活性氧(reactive oxygen species,ROS)水平;钼酸铵法测定过氧化氢酶(catalase,CAT)活性;单细胞凝胶电泳(single-cell gel electrophoresis,SCGE)法检测DNA损伤。[结果]NaAsO2作用L-02肝细胞24h后,50、100、150μmol/L浓度NaAsO2组的L-02肝细胞存活率和CAT活性与对照组相比显著降低(P﹤0.01),且呈剂量-反应关系;100、150μmol/L浓度NaAsO2组ROS生成量显著增多(P﹤0.01);50μmol/L的NaAsO2即可引起出现彗星现象的细胞数明显高于对照组,拖尾细胞阳性率明显升高(P﹤0.01),且呈剂量-反应关系。[结论]NaAsO2对L-02肝细胞的生长具有明显的抑制作用,可引起L-02肝细胞内ROS增多和CAT活性降低,引起细胞DNA损伤。[Objective] To investigate the oxidative injury by NaAsO2 in L-02 hepatic cells. [Methods] L-02 liver cells were treated with NaAsO2 at dose of 50 μmol/L, 100 μmol/L and 150 μmol/L for 24 h. MTT was used to detect the survival rate of L-02 cells with different concentrations of NaAsO2. The level of reactive oxygen species （ROS） was detected by using flow cytometry methods. The catalase （CAT） activity was determined by molybdate ammonium method. The DNA damage was evaluated by single cell gel electrophoresis （SCGE）. [Results] After L-02 liver cells was treated with NaAsO2 for 24 h. Com- pared with the control group, the survival rate and the CAT activity were significantly reduced （P﹤0.01） at dose of 50μmol/L, 100 μmol/L and 150 μmol/L NaAsO2 treated groups. And it showed the dose-response relationship. Excluding low does group, the level of ROS increased significantly （P﹤0.01）; while the number of comet phenomenon was increased when NaAsO2 at 50 μmol/L, rate of trailing cell increased significantly （P﹤0.01） and showed the dose-response relationship. [Conclusion] The survival rate of L-02 hepatic can be inhibited by NaAsO2, NaAsO2 can increase the level of ROS, reduce the CAT activity and also cause DNA damage.

关 键 词：NaAsO2 L-02肝细胞 ROS CAT SCGE DNA损伤 

分 类 号：R994.6[医药卫生—毒理学]