体外培育牛黄复方制剂抗血吸虫病门静脉高压家兔肺组织病变的研究  

Anti-bilharziosis rabbits pulmonary fibrosis of in vitro cultivated calculus bovis compound preparation

作　　者：李涛 杨镇[2] 周正 宋立伟 鲁克宇 蔡红娇[2] 吴在德[2] 

机构地区：[1]煤炭总医院普外科,北京100028 [2]华中科技大学同济医学院附属同济医院外科

出　　处：《中华实验外科杂志》2010年第6期741-743,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（A30170920）

摘　　要：目的 探讨体外培育牛黄复方制剂对血吸虫病门静脉高压肺组织病变干预作用及其机制.方法 采用光镜和电镜方法,观察20例血吸虫病门静脉高压家兔毗喹酮对照组和20例体外培育牛黄复方制剂干预组肺组织的形态学改变;用免疫组织化学技术检测20例血吸虫病门静脉高压家兔毗喹酮对照组和20例体外培育牛黄复方制剂干预组肺绀织的纤维连接蛋向（FN）、层粘连蛋白（LN）的表达.结果 血吸虫病门静脉高压家兔毗喹酮对照组肺组织呈肺泡腔渗出液较多,可见较多巨噬细胞,肺泡毛细血管扩张,毛细血管三层屏障结构模糊,体外培育牛黄复方制剂干预组肺组织部分区域肺泡间隙纤维轻微增多,I型上皮、基底膜、内皮结构基本完整,肺泡腔无明显渗出;血吸虫病门静脉高压家兔毗喹酮对照组肺组织FN、LN表达呈阳性或强阳性,体外培育牛黄复方制剂干预组肺组织FN、LN则呈阴性或弱阳性表达,两者差异有统计学意义（P〈0.01）.结论 体外培育牛黄复方制剂能够有效预防血吸虫病家兔门脉高压症的肺部并发症,它是通过改善肺微循环、降低细胞外基质含量来抑制肺组织病变.Objective To explore the intervention effects and the mechanism of in vitro cultivated calculus bovis compound preparation （ICCBco） on pulmonary lesion in portal hypertensive rabbits with schistosomiasis. Methods The experimental group included 20 portal hypertensive rabbits with schistosomiasis treated by ICCBco. The control group included 20 portal hypertensive rabbits with schistosomiasis treated by praziquantel.Light and electron microscopy were applied to observe the morphological changes of the pulmonary tissues. The expression of Fibronectin （FN） and Laminin （LN） in the lung tissues was detected by immunohistochemistry. Results More alveolar exudation was found in the lung tissue of the praziquantel control group, and more macrophages, alveolar angiotelectasis and the blurred three-tier structure of alveolar-capillary barrier could also be seen. In ICCBco intervention group fibers within the alveolar interspace had a slight increase in some lung regions. The basic integrity structure of type I epithelium, basement membrane and endodermis were basically intact, and there was no obvious exudation from the alveolar space. There was a positive or strong positive expression of FN and LN in the lung tissue of the praziquantel control group, while a negative or weak positive expression of FN and LN in ICCBco intervention group with the difference being significant （P〈0.01）. Conclusion ICCBco can effectively prevent pulmonary complications in portal hypertensive rabbits with schistosomiasis by improving lung microcirculation and lowering the content of extracellular matrix.

关 键 词：体外培育牛黄复方制剂 血吸虫病 门静脉高压症 肺 

分 类 号：R285.5[医药卫生—中药学]